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It is not known whether hepatocytes, a cuboidal epithelium in which transcellular transport is a requisite step in normal apical membrane biogenesis, contain microtubules arranged with a similar polarity. In the present study, we explore the question of microtubule polarity and possible mechanisms for nucleation in the epithelial cell lines WIF\u2010B (hepatocyte), Caco\u20102 (intestine), and Madin\u2010Darby canine kidney (MDCK). Caco\u20102 microtubules in the apicobasal axis had uniform polarity with minus ends nearest the apical surface. After cold and nocodazole\u2010induced depolymerization, microtubule regrowth initiated in the apical region in all three cell types. The apex of WIF\u2010B and Caco\u20102 cells contained two pools of \u03b3\u2010tubulin: one associated with centrosomes and the other delocalized under the apical membrane. Non\u2010centrosomal \u03b3\u2010tubulin was present in complexes that sedimented between 10S and 29S; both forms could bind microtubules. The presence of both centrosomal and noncentrosomal \u03b3\u2010tubulin in apical cytoplasm suggests multiple mechanisms by which microtubule nucleation might occur in epithelial cells. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cm.970320404","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T01:47:13Z","timestamp":1109209633000},"page":"273-288","source":"Crossref","is-referenced-by-count":154,"title":["Polarity and nucleation of microtubules in polarized epithelial cells"],"prefix":"10.1002","volume":"32","author":[{"given":"Tim","family":"Meads","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Trina A.","family":"Schroer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,4]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.1.179"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.125.1.67"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.120.6.1427"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.1.171"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.21.8335"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.109.6.2817"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.105.3.1241"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.84.1.151"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.121.3.485"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/359246a0"},{"key":"e_1_2_1_12_1","first-page":"260","article-title":"Microtubule disruption interferes with the structural and functional integrity of the apical pole in primary cultures of rat hepatocytes","volume":"56","author":"Durand\u2010Schneider A. 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