{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:47:46Z","timestamp":1761918466737,"version":"build-2065373602"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,12,18]],"date-time":"2002-12-18T00:00:00Z","timestamp":1040169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Czech Ministry of Health","award":["6509-3","6406-3"],"award-info":[{"award-number":["6509-3","6406-3"]}]},{"DOI":"10.13039\/100007397","name":"Charles University","doi-asserted-by":"crossref","award":["57"],"award-info":[{"award-number":["57"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Agency of the Czech Republic","award":["301\/P055"],"award-info":[{"award-number":["301\/P055"]}]},{"name":"Czech Ministry of Education Programs","award":["111300001","111300003","111300005"],"award-info":[{"award-number":["111300001","111300003","111300005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer"],"published-print":{"date-parts":[[2003,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The translocation t(12;21)(p13;q22), which produces the TEL\/AML1 fusion gene, is the most frequent chromosomal abnormality in patients with childhood acute lymphoblastic leukemia (ALL) and generally is associated with a favorable prognosis. Furthermore, real\u2010time quantitative\u2010polymerase chain reaction (RQ\u2010PCR)\u2010based detection of TEL\/AML1 represents an accurate technique for the reproducible assessment of minimal residual disease (MRD).<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>The authors employed RQ\u2010reverse transcriptase\u2010PCR (RQ\u2010RT\u2010PCR) technology to analyze MRD levels in 57 newly diagnosed patients with TEL\/AML1 positive ALL in a prospective study.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>On Day + 33, a particularly important time point in terms of outcome prediction based on MRD monitoring, 75% of patients reached negativity, 13% of patients were positive at very low levels (&lt; 10<jats:sup>\u22124<\/jats:sup>; i.e., 1 or more leukemic cell per 10<jats:sup>4<\/jats:sup> normal cells), and another 13% of patients were positive at the level of 10<jats:sup>\u22122<\/jats:sup> to 10<jats:sup>\u22124<\/jats:sup> cells. No patient showed MRD levels \u2265 10<jats:sup>\u22122<\/jats:sup> cells at this time. The data demonstrate that patients with TEL\/AML1 positive ALL had a better response to induction chemotherapy on Day + 33 compared with a group of unselected patients with ALL (<jats:italic>P<\/jats:italic> = 0.0001). However, four patients with TEL\/AML1 positive ALL developed relapse disease. Remarkably, these children were positive for MRD on Day + 33 at a level between 10<jats:sup>\u22122<\/jats:sup> cells and 10<jats:sup>\u22124<\/jats:sup> (<jats:italic>n<\/jats:italic> = 3 patients) and at &lt; 10<jats:sup>\u22124<\/jats:sup> (<jats:italic>n<\/jats:italic> = 1 patient). Kaplan\u2013Meier analysis of disease free survival showed the statistical significance of this distribution (MRD positive vs. MRD negative; log\u2010rank <jats:italic>P<\/jats:italic> = 0.0016).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The authors conclude that, although the TEL\/AML1 positive leukemias generally are associated with a favorable outcome, MRD positivity assessed by RQ\u2010RT\u2010PCR analysis at the end of induction therapy represents a significantly negative prognostic feature. Cancer 2003;97:105\u201313. \u00a9 2003 American Cancer Society.<\/jats:p><jats:p>DOI 10.1002\/cncr.11043<\/jats:p><\/jats:sec>","DOI":"10.1002\/cncr.11043","type":"journal-article","created":{"date-parts":[[2002,12,19]],"date-time":"2002-12-19T08:57:58Z","timestamp":1040288278000},"page":"105-113","source":"Crossref","is-referenced-by-count":30,"title":["Slower molecular response to treatment predicts poor outcome in patients with TEL\/AML1 positive acute lymphoblastic leukemia"],"prefix":"10.1002","volume":"97","author":[{"given":"Jozef","family":"Mad\u017eo","sequence":"first","affiliation":[]},{"given":"Jan","family":"Zuna","sequence":"additional","affiliation":[]},{"given":"Kate\u0159ina","family":"Mu\u017e\u00edkov\u00e1","sequence":"additional","affiliation":[]},{"given":"Mark\u00e9ta","family":"Kalinov\u00e1","sequence":"additional","affiliation":[]},{"given":"Ond\u0159ej","family":"Krej\u010d\u00ed","sequence":"additional","affiliation":[]},{"given":"Ond\u0159ej","family":"Hru\u0161\u00e1k","sequence":"additional","affiliation":[]},{"given":"Berta","family":"Otov\u00e1","sequence":"additional","affiliation":[]},{"given":"Jan","family":"Star\u00fd","sequence":"additional","affiliation":[]},{"given":"Jan","family":"Trka","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,12,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2401258"},{"key":"e_1_2_6_3_2","first-page":"1985","article-title":"TEL\/AML1 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