{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T17:00:09Z","timestamp":1774026009002,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":12001,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1971,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new technique combining microfluorometric DNA assays with differential cell counts was used to quantitate the intralaminar distribution of neuronal and non\u2010neuronal cells (chiefly glial) in rat somatosensory cortex (Charles River 250 gm males, C D\u00ae strain). The intracortical amounts of DNA per unit fresh volume were calculated from the DNA contents of serial frozen slices of known volume sampled serially from the pial surface to white matter in frozen cortical cylinders; respective amounts per unit solids were calculated from predetermined dry weights of the slices. Cell counts were performed on serial horizontal sections from formalin\u2010fixed cylinders stained by Nissl's method. Neuronal DNA and glial DNA were calculated based on the percentages of the respective cells counted.<\/jats:p><jats:p>Total DNA averaged 5.43 \u03bcg\/mg dry weight (1.19 \u03bcg mm<jats:sup>3<\/jats:sup> fresh volume). Values were highest in layers II and IV. Neuronal DNA paralleled total DNA in its intracortical distribution and showed distinct peaks in layers II and IV. Glial DNA showed an even distribution. Glia exceeded neurons only in layers I and VIc. The mean neuron\/glia ratio was 2.5. This method gives a more precise estimate of the absolute numbers of neurons and glia than can be obtained by histological methods alone, since DNA assays eliminate the need to correct the histological counts for volume changes during fixation and staining.<\/jats:p>","DOI":"10.1002\/cne.901430405","type":"journal-article","created":{"date-parts":[[2004,12,28]],"date-time":"2004-12-28T19:44:42Z","timestamp":1104263082000},"page":"481-490","source":"Crossref","is-referenced-by-count":123,"title":["Quantitative cytoarchitectonic distribution of neurons, glia, and DNA in rat cerebral cortex"],"prefix":"10.1002","volume":"143","author":[{"given":"Norman H.","family":"Bass","sequence":"first","affiliation":[]},{"given":"Helen H.","family":"Hess","sequence":"additional","affiliation":[]},{"given":"Alfred","family":"Pope","sequence":"additional","affiliation":[]},{"given":"Caroline","family":"Thalheimer","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090940210"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1090800205"},{"key":"e_1_2_1_4_1","first-page":"1061","article-title":"L'acide desoxyribonucl\u00e9ique du noyau cellulaire depositaire des caract\u00e8res h\u00e9r\u00e9ditaires: Arguments d'ordre analytique","volume":"226","author":"Boivin A.","year":"1948","journal-title":"R. Acad. Sci. Paris"},{"key":"e_1_2_1_5_1","first-page":"1","article-title":"The size of the body and the number of the nerve cells in the cerebral cortex","volume":"3","author":"Bok S. T.","year":"1939","journal-title":"Acta Neurol. Morphol."},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0660651"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-196710000-00006"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091340110"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)61274-1"},{"key":"e_1_2_1_10_1","first-page":"47","article-title":"Method for quantitative morphologic analysis of tissues","volume":"4","author":"Chalkley H. W.","year":"1943","journal-title":"J. Nat. Cancer Inst."},{"key":"e_1_2_1_11_1","first-page":"3","volume-title":"Liver Function","author":"Daoust R.","year":"1958"},{"key":"e_1_2_1_12_1","first-page":"977","article-title":"Neue Untersuchungen uber die Markbildung in den menschichen Grosshirnlappen","volume":"17","author":"Flechsig P.","year":"1898","journal-title":"Neurol. Centrlbl."},{"key":"e_1_2_1_13_1","first-page":"121","volume-title":"Anatomic des menchlichen Gehirns und Ruckenmarks auf myelogenetischer Grundlage","author":"Flechsig P.","year":"1920"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1968.tb11615.x"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000140905"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-196610000-00009"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1152\/ajplegacy.1956.184.2.301","article-title":"Use of deoxyribonucleic acid as a reference standard in metabolic experiments","volume":"184","author":"Gray D. E.","year":"1956","journal-title":"Amer. J. Physiol."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.126.3263.76"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.160.3827.537"},{"key":"e_1_2_1_20_1","first-page":"200","volume-title":"Regional Neurochemistry","author":"Hess H.","year":"1961"},{"key":"e_1_2_1_21_1","first-page":"289","volume-title":"Handbook of Neurochemistry","author":"Hess H. H.","year":"1971"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1965.tb06755.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1971.tb00227.x"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)68051-8"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900840205"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900840302"},{"key":"e_1_2_1_27_1","first-page":"288","volume-title":"Physiology of the Nervous System","author":"Lorente de N\u00f3 R.","year":"1943"},{"key":"e_1_2_1_28_1","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/S0021-9258(18)72054-1","article-title":"A simple quartz torsion balance","volume":"152","author":"Lowry O. H.","year":"1944","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/1.6.420"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(65)90189-9"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39550-X"},{"key":"e_1_2_1_32_1","first-page":"100","volume-title":"Ultra\u2010structure and Cellular Chemistry of Neural Tissue","author":"Nurnberger J. I.","year":"1957"},{"key":"e_1_2_1_33_1","first-page":"419","article-title":"An electron microscope study of the distribution and patterns of astroglial processes in the central nervous system","volume":"99","author":"Peters A.","year":"1965","journal-title":"J. Anat. (london)"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1952.15.2.115"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1956.tb12054.x"},{"key":"e_1_2_1_36_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1016\/0926-6550(63)90339-6","article-title":"Studies on the estimation of deoxyribonucleic acid and ribonucleic acid in rat brain","volume":"72","author":"Santen R. J.","year":"1963","journal-title":"Biochem. biophys. Acta"},{"key":"e_1_2_1_37_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1093\/oxfordjournals.jbchem.a128261","article-title":"Preparation and characterization of nerve cell perikaryon from rat cerebral cortex","volume":"59","author":"Satake M.","year":"1966","journal-title":"J. Biochem. (tokyo)"},{"key":"e_1_2_1_38_1","first-page":"573","article-title":"Zur Glia\u2010architektonik der menschlichen Groshirnrinde im Nissl\u2010bild. Archiv f\u00fcr Psychiatric und Zeitschrift fur Ges","volume":"199","author":"Schlote W.","year":"1959","journal-title":"Neurologie"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0530460"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520294309105794"},{"key":"e_1_2_1_41_1","first-page":"3","article-title":"Morphological criteria of growth and maturation of the nervous system in man","volume":"39","author":"Yakovlev P.","year":"1962","journal-title":"Res. Publ. Assoc. Nerv. Ment. Dis."}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.901430405","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.901430405","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T05:04:52Z","timestamp":1699851892000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.901430405"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1971,12]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1971,12]]}},"alternative-id":["10.1002\/cne.901430405"],"URL":"https:\/\/doi.org\/10.1002\/cne.901430405","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[1971,12]]}}}