{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T03:07:49Z","timestamp":1768014469525,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":11484,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1973,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The staining of half\u2010micron thick Epon sections with toluidine blue provides a reliable method for the identification of glial cells. The diagnostic features of these cells observed in the <jats:italic>corpus callosum<\/jats:italic> of one\u2010month\u2010old rats are as follows: (1) <jats:italic>Astrocytes<\/jats:italic> have a very pale nucleus and cytoplasm; the nuclear envelope is sharply outlined by a thin chromatin lining with occasional chromatin beads; (2) <jats:italic>Microglia<\/jats:italic> have a small nucleus in which large, dark chromatin masses contrast with the nucleoplasm; the nucleus is round or elongated, and may be somewhat angular. The pericytes, which have a similar but usually crescentic nucleus, may be related to microglia. (3) <jats:italic>Oligodendrocytes<\/jats:italic> vary from pale to very dense and may be arbitrarily classified into three subgroups: The <jats:italic>light oligodendrocytes<\/jats:italic> are large pale cells with round to ovoid nucleus containing a prominent nucleolus and little or no condensed chromatin; the cytoplasm is extensive and appears pale, although less so than the nucleus. The <jats:italic>medium shade oligodendrocytes<\/jats:italic> are smaller cells that have an ovoid nucleus carrying small chromatin clumps and appearing moderately basophilic throughout; the cytoplasm is less extensive and somewhat darker than in the \u201clight\u201d subgroup. The <jats:italic>dark oligodendrocytes<\/jats:italic> are usually smaller than medium shade cells and often have an indented or angular nucleus with large chromatin masses; both nucleoplasm and cytoplasm are densely stained; the cytoplasm is often scanty and accumulated on one side of the nucleus.<\/jats:p><jats:p>Semithin sections of the <jats:italic>cerebral cortex<\/jats:italic> show glia with similar features, with the exception of the astrocytes, which have a smaller but more basophilic cytoplasm than in corpus callosum.<\/jats:p><jats:p>The cells of the <jats:italic>subependymal layer<\/jats:italic> of the lateral ventricle, when examined in semithin sections in the neighborhood of the corpus callosum, display a patchy, irregular nucleus and a scanty cytoplasm. Similar cells with a more regular nucleus are found outside the layer and in the corpus callosum; they are designated <jats:italic>free subependymal cells<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/cne.901490104","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T15:29:44Z","timestamp":1104593384000},"page":"43-71","source":"Crossref","is-referenced-by-count":312,"title":["Investigation of glial cells in semithin sections. I. Identification of glial cells in the brain of young rats"],"prefix":"10.1002","volume":"149","author":[{"given":"E. A.","family":"Ling","sequence":"first","affiliation":[]},{"given":"Jean A.","family":"Paterson","sequence":"additional","affiliation":[]},{"given":"A.","family":"Privat","sequence":"additional","affiliation":[]},{"given":"S.","family":"Mori","sequence":"additional","affiliation":[]},{"given":"C. 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