{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T14:50:21Z","timestamp":1770216621271,"version":"3.49.0"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":10235,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1976,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Electron microscopy and <jats:sup>3<\/jats:sup>H\u2010thymidine autoradiographic techniques were used to study the fine structure of proliferating cells in developing rat optic nerve. Before the closure of the optic canal almost all of the cells incorporating radioactive thymidine are ventricular cells, but after closure (16 days of gestation) the vast majority are differentiating astroblasts or oligodendroblasts. Labeled astroblasts show a range in their degree of differentiation; some cells lack 90 \u00c5 cytoplasmic filaments while others have glial filaments and abundant cytoplasmic organelles. In contrast to astroblasts, all of the labeled oligodendroblasts are in the early stages of differentiation. The proliferation of oligodendroblasts starts at five days postnatal, approximately a day or two before the onset of myelination. During myelinogenesis a few of the labeled oligodendroblasts show presumptive connections to myelin sheaths. Microglial cells do not appear to play a major role in gliogenesis since they form less than 2% of all the labeled cells.<\/jats:p><jats:p>The results of this study indicate that astroblasts and oligodendroblasts, rather than undifferentiated glioblasts, are the major source of macroglia. The finding that proliferating glia are in the process of differentiation agrees with recent studies which show that differentiated cells can divide.<\/jats:p>","DOI":"10.1002\/cne.901690303","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T16:24:18Z","timestamp":1104596658000},"page":"291-311","source":"Crossref","is-referenced-by-count":261,"title":["Electron microscopic autoradiographic studies of gliogenesis in rat optic nerve I. Cell proliferation"],"prefix":"10.1002","volume":"169","author":[{"given":"Robert P.","family":"Skoff","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald L.","family":"Price","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adelaine","family":"Stocks","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/7.3.153"},{"key":"e_1_2_1_3_1","volume-title":"Developmental Anatomy","author":"Arey L. B.","year":"1954"},{"key":"e_1_2_1_4_1","first-page":"581","article-title":"The relationship of the cell cycle to tumor growth and control of cell division: a review","volume":"25","author":"Baserga R.","year":"1965","journal-title":"Cancer Res."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(69)90259-5"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00355032"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.10.1.67"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901330104"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-017917-9.50033-0"},{"key":"e_1_2_1_10_1","first-page":"69","article-title":"El tercer elemente de los centros nerviosos","volume":"9","author":"del Rio\u2010Hortega P.","year":"1919","journal-title":"Bol. Soc. Exp. de Biol."},{"key":"e_1_2_1_11_1","first-page":"23","article-title":"Son homologables la glia de escasas radiaciones y la celula de Schwann","volume":"10","author":"del Rio\u2010Hortega P.","year":"1922","journal-title":"Bol. Soc. Exp. Biol."},{"key":"e_1_2_1_11_2","first-page":"1","volume":"7","journal-title":"Arch. Histol. Nor. Patol."},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/B978-0-12-395618-7.50006-8","volume-title":"Major Problems in Developmental Biology","author":"Ebert J. D.","year":"1966"},{"key":"e_1_2_1_13_1","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1007\/BF00342215","article-title":"Electron microscopic studies on the differentiation of the ependymal cells and the glioblast in the spinal cord of domestic fowl","volume":"64","author":"Fujita H.","year":"1964","journal-title":"Z. Zellforsch."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.33.3.645"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-134-34936"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01097190"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00391127"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.66.3.663"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901500205"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901600405"},{"key":"e_1_2_1_21_1","first-page":"135","article-title":"Configurazione dell'oligodendroglia interfascicolare e sul omogia colla cellula dello Schwann","volume":"52","author":"Loreti F.","year":"1938","journal-title":"Riv. Pat. Nerv. Ment."},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.1939.tb07366.x"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1007\/BF00582925","article-title":"Further differentiation of early neural tube explants in organ culture as studied by electron microscopy","volume":"168","author":"Lyser K. M.","journal-title":"Wilhelm Roux' Archiv."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00685066"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1002\/cne.901350104","article-title":"Identification of microglia in light and electron microscopy","volume":"135","author":"Manuelidis E. E.","year":"1969","journal-title":"J. Comp. Neur."},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/cne.901370206","article-title":"Electron microscopic features and proliferation of astrocytes in the corpus callosum of the rat","volume":"137","author":"Manuelidis E. E.","year":"1969","journal-title":"J. Comp. Neur."},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/cne.901390102","article-title":"Electron microscopic identification of three classes of oligodendrocytes and a preliminary study of their proliferative activity in the corpus callosum of young rats","volume":"139","author":"Manuelidis E. E.","year":"1970","journal-title":"J. Comp. Neur."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/88.5.937"},{"key":"e_1_2_1_29_1","volume-title":"The Fine Structure of the Nervous System","author":"Peters A.","year":"1970"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00306691"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(72)90083-8"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(71)90108-4"},{"key":"e_1_2_1_33_1","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1111\/j.1471-4159.1975.tb11657.x","article-title":"The isolation and characterization of a plasma membrane and a myelin fraction derived from oligodendroglia of calf brain","volume":"24","author":"Poduslo S. E.","year":"1974","journal-title":"J. Neurochem."},{"key":"e_1_2_1_34_1","first-page":"27","volume-title":"Progress in Neuropathology","author":"Raine C.","year":"1973"},{"key":"e_1_2_1_35_1","first-page":"253","article-title":"Dispositions morphologiques des cellules de Schwann qui viennent a l'appui de l'hipothese de l'holologie avec les oligodendrocytes","volume":"29","author":"Raine C.","year":"1934","journal-title":"Trab. Lab. Invest. Biol."},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.53.2.587"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901350107"},{"key":"e_1_2_1_38_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1159\/000143978","article-title":"Autoradiographic and Feulgen reaction study of the regenerating basal melanocytes of the white guinea pig","volume":"85","author":"Shukla R. C.","year":"1973","journal-title":"Acta Anat."},{"key":"e_1_2_1_39_1","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1007\/978-3-642-85986-1_12","volume-title":"Contemporary Research Methods Neuroanatomy","author":"Sidman R. L.","year":"1970"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901610408"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901530202"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901690304"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901410202"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00220217"},{"key":"e_1_2_1_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/246110a0"},{"key":"e_1_2_1_46_1","first-page":"37","article-title":"Histogenesis of the anterior limb of the anterior commissure of the mouse brain III. An electron microscopic study of gliogenesis","volume":"117","author":"Sturrock R. R.","year":"1974","journal-title":"J. Anat."},{"key":"e_1_2_1_47_1","first-page":"223","article-title":"A light and electron microscopic study of proliferation and maturation of fibrous astrocytes in the optic nerve of the human embryo","volume":"119","author":"Sturrock R. R.","year":"1975","journal-title":"J. Anat."},{"key":"e_1_2_1_48_1","volume-title":"Human Neuroanatomy","author":"Truex R. C.","year":"1969"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901560102"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01098730"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00319179"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901400204"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/aja.1001210109"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901330207"},{"key":"e_1_2_1_55_1","first-page":"39","volume-title":"Structure and Function of Nervous Tissue","author":"Vaughn J. E.","year":"1972"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.47.1.310"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.57.1.175"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.901690303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.901690303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T04:42:47Z","timestamp":1699850567000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.901690303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1976,10]]},"references-count":57,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1976,10]]}},"alternative-id":["10.1002\/cne.901690303"],"URL":"https:\/\/doi.org\/10.1002\/cne.901690303","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[1976,10]]}}}