{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T03:16:44Z","timestamp":1771039004391,"version":"3.50.1"},"reference-count":68,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":9597,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1978,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microglial cells are absent from the corpus callosum of newborn rats. In the hope of finding out when and how microglial cells appear with age, <jats:sup>3<\/jats:sup>H\u2010thymidine was given intraperitoneally as single or three shortly spaced injections to 5\u2010day\u2010old rats weighing about 15 g; and these animals were sacrificed at various time intervals from 2 hours to 35 days later. Pieces of corpus callosum were taken near the superior lateral angle of the lateral ventricles; and semithin sections were radioautographed and stained with toluidine blue.<\/jats:p><jats:p>The corpus callosum of 5\u2010day\u2010old rats is composed of loosely arranged unmyelinated fibers and scattered cells. Among these cells, microglia are rare; there are a few astrocytes, many immature glial cells, rare pericytes, and 6\u20107% of phagocytic \u201cameboid cells\u201d consisting of a few monocytes and many macrophages. In the animals sacrificed two hours after <jats:sup>3<\/jats:sup>H\u2010thymidine administration, label is present only in immature cells and \u201cameboid cells.\u201d<\/jats:p><jats:p>As time elapses and the fibers of corpus callosum become myelinated, oligodendrocytes and, later, microglial cells appear. At the age of 12 days, microglial cells are present in substantial number; and by 19 days, the number doubles to reach a plateau. Many of the new microglial cells are labeled, e.g., 78.1% in 12\u2010day\u2010old animals (7 days after <jats:sup>3<\/jats:sup>H\u2010thymidine administration). The labeled microglial cells must have come from the transformation of cells that acquired label early, that is, from the immature cells or the \u201cameboid cells.\u201d The height of the peaks of labeling \u2013 59.8% at nine days for immature cells and 77.8% at 12 days for \u201cameboid cells\u201d \u2013 points to the latter as precursors of the highly labeled microglial cells. Furthermore, the \u201cameboid cells\u201d disappear as microglial cells appear and there are transitional elements between these two cell types. Cell counts suggest that about a third of the \u201cameboid cells\u201d transform into microglial cells, while the others degenerate and die.<\/jats:p><jats:p>Thus, the microglial cells which appear in the corpus callosum during the first three weeks of life result from transformation of the \u201cameboid cells\u201d \u2013 a group of macrophages showing various stages of transition from monocytes. As for the occasional microglial cell appearing after the third week or in the adult, they presumably come directly from monocytes. In either case, monocytes would be the intial precursors.<\/jats:p>","DOI":"10.1002\/cne.901800109","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T18:26:42Z","timestamp":1104604002000},"page":"139-163","source":"Crossref","is-referenced-by-count":137,"title":["Radioautographic investigation of gliogenesis in the corpus callosum of young rats II. Origin of microglial cells"],"prefix":"10.1002","volume":"180","author":[{"given":"Kikuko","family":"Imamoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. P.","family":"Leblond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1002\/cne.901510102","article-title":"Cell proliferation in injured spinal cord. 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