{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:13:09Z","timestamp":1761016389803},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":7263,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1984,11,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The response of dorsal root ganglia (DRG) neurons to NGF deprivation and to axotomy was examined in adult guinea pigs. The success of NGF deprivation by means of an autoimmune approach was monitored by the measurement of serum antibody titer levels against guinea pig NGF with the standard bioassay for NGF activity. That the antibody produced NGF deprivation was confirmed by histologic evidence of neuronal atrophy and apparent cell loss in sections of the superior cervical ganglia (SCG) and by marked decreases (65\u201380%) of SCG neurotransmitter\u2010synthesizing enzyme activity levels. By using the autoimmune approach a new source of guinea pigs was found which consistently produced high titers of cross\u2010reacting anti\u2010NGF antibodies.<\/jats:p><jats:p>Experiments were designed to examine the response of the sensory neuron to injury while chronically deprived of NGF. Total neuronal counts in the sixth lumbar DRG 98 days after sciatic nerve crush showed no difference between NGF\u2010deprived and control ganglia. Measurement of the size spectrum of DRG neurons showed evidence of atrophy of the NGF\u2010deprived neurons in both the uninjured and axotomized side compared to respective controls. The mean volume of uninjured sensory neurons measured in the NGF\u2010deprived guinea pigs was decreased 27.7% (P &lt;0.05) compared with that of control guinea pigs.<\/jats:p><jats:p>The degree of regeneration 6 days following a nerve crush was the same in NGF\u2010deprived sensory neurons and in controls when measured by the \u201cpinch test\u201d and by isotope\u2010labeled axonal transport studies. The long\u2010term regenerative response was analyzed with various histologic and morphometric techniques studying the sural nerve 98 days after sciatic nerve crush. No differences between control and NGF\u2010deprived guinea pig sural nerves were noted at the light microscopic and ultrastructural levels. These studies demonstrated an unimpaired regenerative capability of sensory neurons deprived of NGF compared with controls.<\/jats:p>","DOI":"10.1002\/cne.902300110","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T19:19:15Z","timestamp":1104607155000},"page":"110-118","source":"Crossref","is-referenced-by-count":124,"title":["Role of nerve growth factor in the adult dorsal root ganglia neuron and its response to injury"],"prefix":"10.1002","volume":"230","author":[{"given":"Keith M.","family":"Rich","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henry K.","family":"Yip","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia A.","family":"Osborne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Eugene M.","family":"Johnson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/291413a0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00221931"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.64.2.787"},{"key":"e_1_2_1_5_1","first-page":"329","volume-title":"Translator: Degeneration and Regeneration of the Nervous System","author":"Cajal S. 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