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Computer\u2010assisted reconstructions from serial sections were utilized to aid in visualization of taste bud cell\u2010nerve fiber synapses.<\/jats:p><jats:p>We have classified taste bud cells on the basis of previously established criteria\u2010namely, size of the nucleus, shape and density of chromatin, density of cytoplasm, and presence or absence of dense\u2010cored or clear vesicles, other cytoplasmic organelles, and synaptic foci. Both dark cells and light cells are present, as well as cells with intermediate morphological characteristics.<\/jats:p><jats:p>Synapses were observed from taste bud cells onto nerve fiber processes. In virtually all instances, synapses are associated with the nuclear region of the taste cell. These synapses are characterized by the presence of 40\u201370 nm clear vesicles embedded in a thickened presynaptic membrane separated from the postsynaptic membrane by a 16\u201330 nm cleft. Synapses are not unique to any particular cell type. Dark, intermediate, and light cells all synapse onto nerve fibers. Two general types of synapses exist: spot (or macular) and fingerlike. In the latter, the postsynaptic region of the neuronal process protrudes into an invagination of the taste cell membrane. Differences in synaptic morphology are not correlated with taste cell type. In some cases a single taste cell was observed to possess both macular and fingerlike synapses adjacent to one another, forming a synaptic complex onto a single neuronal process. On the basis of the presence of synaptic contacts, we conclude that both \u201cdark\u201d and \u201clight\u201d cells are gustatory receptors.<\/jats:p>","DOI":"10.1002\/cne.902350105","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T19:30:06Z","timestamp":1104607806000},"page":"48-60","source":"Crossref","is-referenced-by-count":143,"title":["Ultrastructure of mouse vallate taste buds. I. 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