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As early as 10 days of age, CGRP\u2010like immunoreactivity was reduced in laminae I, II, and V, as well as in the bundles of fibers situated dorsal and ventral to the central canal. However, beginning on or about 6 weeks of age, the density of CGRP\u2010like immunoreactivity in the superficial laminae and in the bundles dorsal and ventral to the central canal increased. Moreover, thick, nonvaricose CGRP\u2010like immunoreactive fibers appeared in laminae III and IV. These recurring fibers were of primary afferent origin as demonstrated by their disappearance after multiple, unilateral rhizotomies. A similar age\u2010dependent alteration in the density of FRAP activity was also observed. Although virtually absent at 10 days of age after neonatal administration of capsaicin, the density of FRAP activity increased in lamina II by 8 weeks of age. This activity disappeared after multiple, unilateral rhizotomies, indicating that the FRAP activity that reappeared was of primary afferent origin. Neonatal administration of capsaicin also reduced the density of SP\u2010like immunoreactivity in the dorsal horn as early as 10 days of age, although the density of SP\u2010like immunoreactivity showed some recovery after 6 weeks of age. However, unlike CGRP\u2010like immunoreactivity or FRAP activity, the density of SP\u2010like immunoreactivity in capsaicin\u2010treated rats was not detectably altered by multiple, unilateral rhizotomies, indicating that it originated principally from intrinsic dorsal horn neurons. Age\u2010dependent alterations in both thermal and mechanical, but not chemical, nociceptive thresholds were also observed in these same animals. Thus, tail flick latency, hot plate latency, and paw withdrawal threshold were maximally increased at 6 weeks of age, after which time thresholds declined to vehicle\u2010treated values. In contrast, capsaicin\u2010treated animals were uniformly insensitive to ophthalmic administration of capsaicin. The correspondence between developmental alterations in CGRP\u2010like immunoreactivity or FRAP activity and in thermal and mechanical nociceptive thresholds is suggestive of a role of CGRP\u2010 or FRAP\u2010containing primary afferents in thermal and mechanical nociception.<\/jats:p>","DOI":"10.1002\/cne.903120310","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T22:43:59Z","timestamp":1104619439000},"page":"436-450","source":"Crossref","is-referenced-by-count":82,"title":["Developmental alterations in nociceptive threshold, immunoreactive calcitonin gene\u2010related peptide and substance P, and fluoride\u2010resistant acid phosphatase in neonatally capsaicin\u2010treated rats"],"prefix":"10.1002","volume":"312","author":[{"given":"Donna L.","family":"Hammond","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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