{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T22:55:12Z","timestamp":1762296912586},"reference-count":74,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":4452,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1992,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An antiserum was raised in rabbits immunized with octopamine conjugated to thyroglobulin. The specificity of this antiserum for octopamine is shown by dot blot immunoassay analysis. The antiserum does not crossreact with dopamine, noradrenaline, and serotonin, but slight crossreactivity with the amine tyramine at high concentrations was observed. The tyramine crossreactivity could be eliminated by preabsorption with a tyramine\u2010glutaraldehyde\u2010BSA conjugate. Using this antiserum, we describe the topographical distribution of octopamine\u2010immunoreactive (ir) neuronal elements in wholemounts and paraffin sections of the ventral nerve cord of the American cockroach. The pattern of octopamine immunostaining is completely different from that obtained with an antidopamine serum, and can be blocked by preabsorbing the antioctopamine serum with BSA\u2010conjugated octopamine.<\/jats:p><jats:p>Cell bodies and dendritic processes of putatively octopaminergic dorsal (DUM) and ventral (VUM) unpaired median neurons were clearly octopamine\u2010ir in all ganglia examined. The numbers of stained DUM somata in the mesothoracic, metathoracic, and terminal ganglion of females correspond to those of peripherally projecting DUM cells revealed previously by retrograde tracing (Gregory, Philos Trans R Soc Lond [Biol] 306:191, 1984; Tanaka and Washio, Comp Biochem Physiol 91A:37, 1988; Stoya et al., Zool Jb Physiol 93:75, 1989). In addition, various, previously unknown, paired cells with octopamine\u2010like immunoreactivity were found in all ventral ganglia except abdominal ganglia 3\u20136. Some of these probably project intersegmentally. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.903220102","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T23:05:25Z","timestamp":1104620725000},"page":"1-15","source":"Crossref","is-referenced-by-count":81,"title":["A new specific antibody reveals octopamine\u2010like immunoreactivity in cockroach ventral nerve cord"],"prefix":"10.1002","volume":"322","author":[{"given":"Manfred","family":"Eckert","sequence":"first","affiliation":[]},{"given":"J\u00fcrgen","family":"Rapus","sequence":"additional","affiliation":[]},{"given":"Asja","family":"N\u00fcrnberger","sequence":"additional","affiliation":[]},{"given":"Heinz","family":"Penzlin","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"3","volume-title":"Cockroaches as Models for Neurobiology: Application in Biomedical Research","author":"Adams M. 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