{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T21:35:45Z","timestamp":1770500145378,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,10,9]],"date-time":"2004-10-09T00:00:00Z","timestamp":1097280000000},"content-version":"vor","delay-in-days":3616,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1994,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Previous molecular cloning studies have revealed that \u03b1\u2010bungarotoxin binding proteins present in the brain are members of the neuronal nicotinic acetylcholine receptor gene family. The \u03b17 subunit is structurally related to the agonist binding subunits present in the central and peripheral nervous systems and, when expressed in <jats:italic>Xenopus<\/jats:italic> oocytes, forms functional channels blockable by \u03b1\u2010bungarotoxin. In the present study, three different monoclonal antibodies raised against the \u03b17 subunit were used to map its distribution throughout the central nervous system of the rat.<\/jats:p><jats:p>Immunohistochemical localization revealed that the \u03b17 subunit is expressed in most regions of the brain, being, overall, well correlated with previous \u201cin situ\u201d localization of \u03b17 transcripts and \u03b1\u2010bungarotoxin autoradiographic binding studies. Particularly strong immuno\u2010reactivity was observed in several sensory and motor nuclei of the brainstem as well as the red nucleus. At the cellular level, \u03b17 immunostaining was usually found both in somata and dendrites, whereas axonal and terminal labeling was not observed.<\/jats:p><jats:p>The widespread distribution of the \u03b17 subunit polypeptide is consistent with immunoprecipitation data demonstrating that it is a component of the predominant subtype of brain \u03b1\u2010bungarotoxin\u2010sensitive nicotinic receptors. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.903490302","type":"journal-article","created":{"date-parts":[[2005,1,1]],"date-time":"2005-01-01T23:19:37Z","timestamp":1104621577000},"page":"325-342","source":"Crossref","is-referenced-by-count":216,"title":["Immunocytochemical localization of the \u03b17 subunit of the nicotinic acetylcholine receptor in the rat central nervous system"],"prefix":"10.1002","volume":"349","author":[{"given":"Eduardo Dominguez","family":"del Toro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose M.","family":"Juiz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon","family":"Lindstrom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Criado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,10,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/10799899109064693"},{"key":"e_1_2_1_3_1","first-page":"1455","article-title":"Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons I. 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