{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T00:27:26Z","timestamp":1773448046173,"version":"3.50.1"},"reference-count":97,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,10,10]],"date-time":"2004-10-10T00:00:00Z","timestamp":1097366400000},"content-version":"vor","delay-in-days":3268,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Comparative Neurology"],"published-print":{"date-parts":[[1995,10,30]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Systemic interleukin\u20101 (IL\u20101) activates the hypothalamo\u2010pituitary\u2010adrenal (HPA) axis, an effect exerted through increased synthesis and secretion of corticotropin\u2010releasing factor (CRF) by parvicellular neurosecretory neurons. The site(s) and mechanism(s) through which circulat ing IL\u20101 may access central systems governing HPA axis output remain obscure. To identify potential cellular targets for blood\u2010borne IL\u20101, we analyzed the distribution of mRNA encoding the rat type 1 IL\u20101 receptor (IL\u20101R1) in rat brain. Regional ribonuclease protection assays detected a single protected fragment corresponding to the membrane\u2010bound form of the IL\u20101R1 mRNA in all areas analyzed. In situ hybridization revealed labeling predominantly over barrier\u2010related cells, including the leptomeninges, non\u2010tanycytic portions of the ependyma, the choroid plexus, and vascular endothelium. Low to moderate levels of the IL\u20101111 mRNA were detected in just a few neuronal cell groups, including the basolateral nucleus of the amygdala, the arcuate nucleus of the hypothalamus, the trigeminal and hypoglossal motor nuclei, and the area postrema. No specific labeling for IL\u20101R1 mRNA was detected over neurons that respond to intravenous IL\u20101\u03b2; by induction of transcription factor Fos, including hypophysiotropic CRF cells and brainstem catecholamine neurons. Injection of IL\u20101\u03b2 did, however, provoke induction of mRNA encoding the immediate\u2010early gene, NGFI\u2010B, but not<jats:italic>c<\/jats:italic>\u2010<jats:italic>fos<\/jats:italic>, in two major loci of IL\u20101R1 expression, vascular endothelial cells, and the area postrema. Intravenous inection of IL\u20101\u03b2 acutely down\u2010regulated IL\u20101R1 mRNA in perivascular cells, but not in neuronal cell groups. These results suggest the parenchymal sites of IL\u20101R1 expression in rat to be distinct from those reported previously in mouse. The common expression in both species of an IL\u20101R in non\u2010neuronal elements highlights the possibility that IL\u20101\u2010mediated activation of CRF neurons may result from cytokine\u2010receptor interaction at vascular, and\/or other barrier\u2010related, sites to trigger release of secondary signalling molecules in a position to interact with components of HPA control circuitry. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cne.903610410","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T21:55:16Z","timestamp":1117230916000},"page":"681-698","source":"Crossref","is-referenced-by-count":385,"title":["Type 1 interleukin\u20101 receptor in the rat brain: Distribution, regulation, and relationship to sites of IL\u20101\u2013induced cellular activation"],"prefix":"10.1002","volume":"361","author":[{"given":"A.","family":"Ericsson","sequence":"first","affiliation":[]},{"given":"C.","family":"Liu","sequence":"additional","affiliation":[]},{"given":"R. P.","family":"Hart","sequence":"additional","affiliation":[]},{"given":"P. E.","family":"Sawchenko","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2004,10,10]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(85)90730-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90412-H"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(89)90185-8"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90893-G"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(93)90053-2"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2443979"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2821620"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3014662"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(91)90284-C"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)62360-2"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-08-04951.1994"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00312822"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(89)90108-2"},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"5126","DOI":"10.1523\/JNEUROSCI.13-12-05126.1993","article-title":"A comparison of two immediate early genes, c\u2010foe and NGFI\u2010B, as markers for functional activation in stress\u2010related neuroendocrine circuitry","volume":"13","author":"Chan R. K. W.","year":"1993","journal-title":"J. Neurosci."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.903480309"},{"key":"e_1_2_1_17_1","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single\u2010step method of RNA isolation by acid\u2010guanidium thiocyanate\u2010phenol\u2010chloroform extraction","volume":"162","author":"Chomczynski P.","year":"1987","journal-title":"Ann. Biochem."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000126215"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(88)90883-9"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90217-8"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(94)90087-6"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90281-O"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-03-01101.1992"},{"key":"e_1_2_1_24_1","first-page":"79","article-title":"Isolation and characterization of the c\u2010fos (rat) cDNA and the analysis of posttranslational modification in vitro","volume":"2","author":"Curran T.","year":"1987","journal-title":"Oncogene"},{"key":"e_1_2_1_25_1","doi-asserted-by":"crossref","first-page":"1657","DOI":"10.4049\/jimmunol.149.5.1657","article-title":"Pattern of IL\u20101 receptor gene expression suggests role in noninflammatory procauses","volume":"149","author":"Deyerle K. L.","year":"1992","journal-title":"J. Immunol."},{"key":"e_1_2_1_26_1","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1182\/blood.V77.8.1627.1627","article-title":"Interleukin\u20101 and interieukin\u20101 antagonism","volume":"77","author":"Dinarello C.","year":"1991","journal-title":"Blood"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(89)90150-7"},{"key":"e_1_2_1_28_1","first-page":"95","article-title":"Distribution of the type\u20101 interieukin\u20101 receptor mRNA in the central nervous system of the rat","volume":"19","author":"Ericsson A.","year":"1993","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.14-02-00897.1994"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/B978-0-12-185281-8.50015-6","article-title":"e\u2010fos\u2010based functional mapping of central pathways subserving the effects of interieukin\u20101 on the hypothalamo\u2010pituitary\u2010adrenal axis","volume":"16","author":"Ericsson A.","year":"1993","journal-title":"Methods Neurosci."},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/334698a0"},{"key":"e_1_2_1_32_1","first-page":"956","article-title":"Biotinylated interieukin\u20101 receptor antagonist (IL\u2010IRA) labels paraganglia in the rat liver hilus and hepatic vagus","volume":"20","author":"Goehler L. E.","year":"1994","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_1_33_1","first-page":"196","article-title":"Brain interleukin 1 receptors: Characterization and modulation after liposaa charide injection","volume":"3","author":"Haour F. G.","year":"1990","journal-title":"Prog. Neuro. Endocr. Immunol."},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1330349"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(93)90267-3"},{"key":"e_1_2_1_36_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1093\/oso\/9780195051063.003.0014","volume-title":"Central Regulations of Autonomic Functions","author":"Johnson A. K.","year":"1990"},{"key":"e_1_2_1_37_1","first-page":"E163","article-title":"Involvement of organum vasculosum of lamina terminalis and preoptic area in interleukin 1 beta\u2010induced ACTH release","volume":"258","author":"Katsuura G.","year":"1990","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_38_1","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S0006-291X(88)80805-2","article-title":"Identification of a high\u2010affinity receptor for interieukin\u20101 beta in rat brain","volume":"156","author":"Katsuura G.","year":"1888","journal-title":"Biochem. Biophys. Res. Comm."},{"key":"e_1_2_1_39_1","doi-asserted-by":"crossref","first-page":"1773","DOI":"10.1210\/endo-122-5-1773","article-title":"Adrenocorticotropin release induced by intracerebroventricular injection of recombinant human interieukin\u20101 in rats: Possible involvement of prostaglandin","volume":"122","author":"Katsuura G.","year":"1888","journal-title":"Endocrinology"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/82.9.772"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(84)90625-5"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.13-02-00744.1993"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.24.11182"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(88)90025-1"},{"key":"e_1_2_1_45_1","first-page":"45","article-title":"A soluble IL\u20101 receptor is expressed in cultured rat sympathetic ganglia from the type 1 IL\u20101 receptor gene","volume":"19","author":"Liu C.","year":"1993","journal-title":"Soc. Neurosci. Abstr."},{"key":"e_1_2_1_46_1","unstructured":"Liu C. andR. P.Hart(1995) Rat type 1 interleukin\u20101 receptor gene is alternatively expressed as a soluble receptor.J. Biol. Chem.submitted."},{"key":"e_1_2_1_47_1","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1093\/oso\/9780195051063.003.0006","volume-title":"Central Regulation of Autonomic Functions","author":"Loewy A. D.","year":"1990"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/0196-9781(91)90015-H"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8424175"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-127-5-2175"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1159\/000126269"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(92)90131-4"},{"key":"e_1_2_1_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(88)90138-9"},{"key":"e_1_2_1_54_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.14.030191.002225"},{"key":"e_1_2_1_55_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1989.sp017571"},{"key":"e_1_2_1_56_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018213"},{"key":"e_1_2_1_57_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.901640202"},{"key":"e_1_2_1_58_1","first-page":"93","article-title":"Establishment of dye\u2010uptake method (A375 assay) for quantitative measurement of IL\u2010 1: Correlation with LAF assay","volume":"69","author":"Nakano K.","year":"1988","journal-title":"Dev. Biol."},{"key":"e_1_2_1_59_1","first-page":"236","article-title":"The afferent discharges from sensors for interleukin l\u03b2 in the hepato\u2010portal system in the rat","volume":"446","author":"Nijima A.","year":"1992","journal-title":"J. Physiol."},{"key":"e_1_2_1_60_1","first-page":"R914","article-title":"Effects of \u03b1\u2010MSH on sleep, behaviour and brain temperatures","volume":"255","author":"Opp M. R.","year":"1988","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9384(90)90343-3"},{"key":"e_1_2_1_62_1","volume-title":"The Fine Structure of the Nervous System: Neurons and Their Supporting Cells","author":"Peters A.","year":"1991"},{"key":"e_1_2_1_63_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/S0149-7634(05)80001-6","article-title":"Immunoregulators in the nervous system","volume":"15","author":"Plats\u2010Salam\u00e1n C. R.","year":"1991","journal-title":"Neurosci. Biobehav. Rev."},{"key":"e_1_2_1_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-0115(86)90022-4"},{"key":"e_1_2_1_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1991.tb01583.x"},{"key":"e_1_2_1_66_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-129-4-2049"},{"key":"e_1_2_1_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(92)91424-D"},{"key":"e_1_2_1_68_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-125-6-2800"},{"key":"e_1_2_1_69_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-125-6-3096"},{"key":"e_1_2_1_70_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3131879"},{"key":"e_1_2_1_71_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_1_72_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2821621"},{"key":"e_1_2_1_73_1","first-page":"55","volume-title":"Stress: Neurobiology and Neuroendocrinology","author":"Sawchenko P. E.","year":"1991"},{"key":"e_1_2_1_74_1","first-page":"15","volume-title":"Stress: Neurochemical and Molecular Approaches","author":"Sawchenko P. E.","year":"1992"},{"key":"e_1_2_1_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-185255-9.50018-3"},{"key":"e_1_2_1_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(82)90280-3"},{"key":"e_1_2_1_77_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-124-6-3131"},{"key":"e_1_2_1_78_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00319366"},{"key":"e_1_2_1_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(88)90346-1"},{"key":"e_1_2_1_80_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1179\/014788889794651870","article-title":"A complete protocol for in situ hybridization of messenger RNAs in brain and other tissues with radiolabeled single\u2010stranded RNA probes","volume":"12","author":"Simmons D. M.","year":"1989","journal-title":"J. Histotechnol."},{"key":"e_1_2_1_81_1","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1994.1100"},{"key":"e_1_2_1_82_1","first-page":"121","article-title":"Passage of immunomodulators across the blood\u2010brain barrier","volume":"63","author":"Stitt J. T.","year":"1990","journal-title":"Yale J. Biol. Med."},{"key":"e_1_2_1_83_1","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1016\/0169-328X(93)90094-6","article-title":"Neuropeptide Y increases the corticotropinreleasing factor messenger ribonucleic acid level in the rat hypothalamus","volume":"18","author":"Suds T.","year":"1993","journal-title":"Mol. Brain Res."},{"key":"e_1_2_1_84_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-1-459"},{"key":"e_1_2_1_85_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.132.4.1497"},{"key":"e_1_2_1_86_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(94)91073-1"},{"key":"e_1_2_1_87_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo-121-4-1580"},{"key":"e_1_2_1_88_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-196707000-00005"},{"key":"e_1_2_1_89_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(87)90176-4"},{"key":"e_1_2_1_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/0361-9230(94)90179-1"},{"key":"e_1_2_1_91_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020055"},{"key":"e_1_2_1_92_1","first-page":"127","volume-title":"Methods in Neurosciences, Genetic Probes","author":"Watts A. G.","year":"1989"},{"key":"e_1_2_1_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/0028-3908(89)90018-X"},{"key":"e_1_2_1_94_1","doi-asserted-by":"publisher","DOI":"10.1159\/000097143"},{"key":"e_1_2_1_95_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(93)90121-5"},{"key":"e_1_2_1_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/0169-328X(94)90180-5"},{"key":"e_1_2_1_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)62359-6"},{"key":"e_1_2_1_98_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830190213"}],"container-title":["Journal of Comparative Neurology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcne.903610410","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cne.903610410","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T05:48:07Z","timestamp":1706248087000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cne.903610410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,10,30]]},"references-count":97,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1995,10,30]]}},"alternative-id":["10.1002\/cne.903610410"],"URL":"https:\/\/doi.org\/10.1002\/cne.903610410","archive":["Portico"],"relation":{},"ISSN":["0021-9967","1096-9861"],"issn-type":[{"value":"0021-9967","type":"print"},{"value":"1096-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,10,30]]}}}