{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T04:17:39Z","timestamp":1773807459810,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":8286,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1982,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cells activated in unidirectional mixed leukocyte cultures (MLC) have been analyzed on the basis of their light scatter characteristics. C57BL\/6 spleen cells were cultured with irradiated (2000 rads) DBA\/2 spleen cells for 5 days and the resulting suspension of activated cells was passed on a FACS II flow cytometer. Correlated parameter analysis of forward light scatter (FLS) and perpendicular light scatter (PLS) indicated that the MLC consisted of a heterogenous mixture of viable cells, dead cells, and subcellular debris. However, by appropriate gating of the FLS\/PLS distribution, viable cells could be identified as a biphasic FLS histogram. Sorting and morphological analyses of these two FLS peaks demonstrated that they corresponded to almost pure populations of small lymphocytes (lower peak) and lymphoblasts (upper peak), respectively. Furthermore, when sorted cells were tested for their ability to lyse antigenically relevant (DBA\/2) tumor target cells in a <jats:sup>51<\/jats:sup>Crrelease assay, lymphoblasts were found to exhibit 40\u2010fold greater cytolytic activity (on a per\u2010cell basis) than small lymphocytes.<\/jats:p>","DOI":"10.1002\/cyto.990030112","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T13:50:20Z","timestamp":1123509020000},"page":"55-58","source":"Crossref","is-referenced-by-count":35,"title":["Light scatter analysis and sorting of cells activated in mixed leukocyte culture"],"prefix":"10.1002","volume":"3","author":[{"given":"H. Robson","family":"MacDonald","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre","family":"Zaech","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.140.3.703"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"490","DOI":"10.4049\/jimmunol.126.2.490","article-title":"Limiting dilution analysis of alloantigen\u2010reactive T lymphocytes. V. Lyt phenotype of cytolytic T lymphocyte precursors reactive against normal or mutant H\u20102 alloantigens","volume":"126","author":"Cerottini JC","year":"1981","journal-title":"J Immunol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/24.1.1254923"},{"key":"e_1_2_1_5_2","first-page":"505","volume-title":"Flow Cytometry and Sorting","author":"Loken MR","year":"1978"},{"key":"e_1_2_1_6_2","first-page":"575","article-title":"Allograft immunity in the mouse. II. Physical studies of the development of cytotoxic effector cells from their immediate progenitors","volume":"111","author":"MacDonald HR","year":"1973","journal-title":"J Immunol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.140.6.1511"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"883","DOI":"10.4049\/jimmunol.126.3.883","article-title":"Flow cytofluorometric analysis of the binding of Vicia villosa lection to T lymphoblasts: lack of correlation with cytolytic function","volume":"126","author":"MacDonald HR","year":"1981","journal-title":"J Immunol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1040730305"},{"key":"e_1_2_1_10_2","first-page":"105","volume-title":"Flow Cytometry and Sorting","author":"Salzman GC","year":"1978"},{"key":"e_1_2_1_11_2","first-page":"19","volume-title":"Experimental Hematology Today","author":"Van den Engh G","year":"1981"},{"key":"e_1_2_1_12_2","first-page":"147","volume-title":"Immunofluorescence and Related Staining Techniques","author":"Visser J","year":"1978"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.152.5.1210"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990030112","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990030112","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T12:17:35Z","timestamp":1699791455000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990030112"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,7]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1982,7]]}},"alternative-id":["10.1002\/cyto.990030112"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990030112","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,7]]}}}