{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T16:51:08Z","timestamp":1773161468490,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":8043,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1983,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new modification of our detergent technique for the preparation of nuclei for flow cytometric DNA analysis is described. The attainment of low coefficients of variation of the peaks and of quantitative staining of nuclei from different tissues was a problem with the original method. This was solved in the new modification by trypsinization of the unfixed nuclei. The nuclei were stabilized by spermine. A simple procedure for long\u2010term storage of samples at \u201480\u00b0C was integrated into the method. The fluorescence of the nuclei was stable for at least 3 hours after staining. Light exposure protection of the samples was essential. No cell loss was caused by storage or staining. The method was successfully applied on samples including: (<jats:italic>a<\/jats:italic>) Normal tissues\u2014 human lymphocytes, granulocytes and spleen. Mouse lymphocytes, bone marrow, spleen, liver, kidney and thymus. (<jats:italic>b<\/jats:italic>) Human neoplasms\u2014 lung cancer, breast cancer, lymphoma, leukemia, bladder cancer and cancer of the oral cavity. (<jats:italic>c<\/jats:italic>) Human tumors in nude mice\u2014 breast cancer, lung cancer, melanoma and colon cancer. (<jats:italic>d<\/jats:italic>) Mouse ascites tumors\u2014 JB\u20101, L 1210, Ehrlich and P 383. It therefore seems well suited as a routine clinical procedure.<\/jats:p>","DOI":"10.1002\/cyto.990030503","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T18:55:25Z","timestamp":1123527325000},"page":"323-327","source":"Crossref","is-referenced-by-count":1218,"title":["A Detergent\u2010trypsin method for the preparation of nuclei for flow cytometric DNA analysis"],"prefix":"10.1002","volume":"3","author":[{"given":"Lars L.","family":"Vindel\u00f8v","sequence":"first","affiliation":[]},{"given":"Ib J.","family":"Christensen","sequence":"additional","affiliation":[]},{"given":"Nis I.","family":"Nissen","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197709)40:3<1206::AID-CNCR2820400334>3.0.CO;2-Z"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1974.tb00252.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(67)90235-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.184.4143.1297"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00496132"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.66.1.188"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(67)90353-1"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990020105"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1007\/BF02889282","article-title":"Flow microfluorometric analysis of nuclear DNA in cells from solid tumors and cell suspensions","volume":"24","author":"Vindel\u00f8v LL","year":"1977","journal-title":"Virchows Arch (Cell Pathol)"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030505"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030502"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990030504"},{"key":"e_1_2_1_14_2","first-page":"2499","article-title":"Treatment of small\u2010cell carcinoma of the lung monitored by sequential flow cytometric DNA analysis","volume":"42","author":"Vindel\u00f8v LL","year":"1982","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_2","first-page":"4295","article-title":"Clonal heterogeneity of small\u2010cell anaplastic carcinoma of the lung demonstrated by flowcytometric DNA analysis","volume":"40","author":"Vindel\u00f8v LL","year":"1980","journal-title":"Cancer Res"},{"key":"e_1_2_1_16_2","first-page":"97","volume-title":"Pulse\u2010Cytophotometry","author":"Zante J","year":"1976"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990030503","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990030503","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T09:16:10Z","timestamp":1697793370000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990030503"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,3]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1983,3]]}},"alternative-id":["10.1002\/cyto.990030503"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990030503","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,3]]}}}