{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T05:47:30Z","timestamp":1770702450971,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":7677,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1984,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The energy transfer efficiency E was measured between fluorescein\u2010conjugated concanavalin A (Con A) and rhodamine\u2010conjugated Con A bound to homogeneous tissue culture cells, the HK22 murine lymphoma cell line. Results from a flow cytometric energy transfer method (FCET) and two different steady\u2010state fluorimeter methods were compared. The data were found to be in close agreement after careful correction of the steady\u2010state fluorimetric measurements for contributions from dissociating ligand. The biological variability of the individual cells with respect to E was calculated using an error propagation analysis and were found to be less than the variability in the absolute amount of ligand binding per cell. FCET has a number of advantages over the fluorimetric measurements using suspensions of cells: (1) relatively labile receptor\u2010ligand complexes can be measured; (2) the analysis can be restricted to undamaged cells by gating the data collection on the light\u2010scattering signals; (3) heterogeneous populations of cells with respect to donor and acceptor topology can be distinguished by the correlation of E with other cellular parameters derived from additional signals or combinations thereof; and (4) the dynamics of donor\u2010acceptor redistribution on subpopulations can be measured.<\/jats:p>","DOI":"10.1002\/cyto.990050216","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:25:09Z","timestamp":1123518309000},"page":"210-216","source":"Crossref","is-referenced-by-count":108,"title":["Fluorescence energy transfer measurements on cell surfaces: A critical comparison of steady\u2010state fluorimetric and flow cytometric methods"],"prefix":"10.1002","volume":"5","author":[{"given":"J\u00e1nos","family":"Sz\u00f6ll\u00f3si","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lajos","family":"Tr\u00f3n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e1ndor","family":"Damjanovich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen H.","family":"Helliwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donna","family":"Arndt\u2010Jovin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas M.","family":"Jovin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/270695a0"},{"key":"e_1_2_1_3_2","first-page":"433","volume-title":"Biophysical Chemistry","author":"Cantor CR","year":"1980"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/27.1.374620"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.19.5985"},{"key":"e_1_2_1_6_2","first-page":"159","volume-title":"Fluorescent Probes","author":"Dale RE","year":"1981"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/264411a0"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01570434"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-9203-7_6"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.6.3469"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/277661a0"},{"key":"e_1_2_1_12_2","first-page":"89","volume-title":"Physical Chemical Aspects of Cell Surface Events in Cellular Regulation","author":"Schlessinger J","year":"1979"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.6.2659"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.94.2.406"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","unstructured":"Tr\u00f3nL Sz\u00f6ll\u00f6siJ DamjanovichS HelliwellSH Arndt\u2010JovinDJ JovinTM:Flow cytometric measurement of fluorescence responance energy transfer on cell surfaces. 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