{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:12:59Z","timestamp":1758816779739},"reference-count":15,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":6216,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1988,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>One of the major drawbacks of droplet sorting in a flow cytometer is the relatively low sorting speed. Thus, we have developed an alternative, faster sorting technique: photodamage cell sorting. In a photodamage cell sorter all unwanted cells, as detected with the first, measuring laser, are killed with the second, damaging laser. Thus, the cells need to be photosensitive to the second laser. In addition, a mechanism is needed to switch this laser on and off based on the sorting criteria. In our photodamage cell sorter, the ZAPPER, we use an acousto\u2010optic crystal to switch the laser beam. Cells are made photosensitive by vital staining with photosensitizers. With cells grown in the presence of 5\u2010bromo\u20102\u2032\u2010deoxyuridine (BrdUrd) and stained with Hoechst 33342 (H42) at least a 5\u2010decade cell reduction is accomplished after irradiation with 400 mW UV light. With this system, sorting rates have been achieved of 30,000 cells per second. Due to the selection based on photodynamic killing, this sorting technique is restricted to the selection of viable cells.<\/jats:p><jats:p>Photodamage cell sorting seems well suited for isolating viable cells occurring in low percentages or for the sorting of large numbers of cells. Another application can be the sorting of large or fragile cells.<\/jats:p>","DOI":"10.1002\/cyto.990090208","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:59:27Z","timestamp":1123516767000},"page":"143-149","source":"Crossref","is-referenced-by-count":17,"title":["High\u2010speed photodamage cell selection using bromodeoxyuridine\/Hoechst 33342 photosensitized cell killing"],"prefix":"10.1002","volume":"9","author":[{"given":"Hans","family":"Herweijer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willem Stokdijk","family":"And","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan W. M.","family":"Visser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/scientificamerican0376-108"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060514"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990050617"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00364015"},{"key":"e_1_2_1_6_2","first-page":"114","article-title":"Photodamage, a basis for super high speed cell selection","volume":"2","author":"Martin JC","year":"1981","journal-title":"Cytometry"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990010507"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990060404"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1982.tb03826.x"},{"key":"e_1_2_1_10_2","volume-title":"Practical Flow Cytometry","author":"Shapiro HM","year":"1985"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01538967"},{"key":"e_1_2_1_12_2","unstructured":"StokdijkW van den EnghGJ van DekkenH:The Rijswijk experimental light\u2010activated cell sorter. International Conference Analytical Cytology XI Hilton Head (S.C.) November 17\u201322 (1985) Abstract 318."},{"key":"e_1_2_1_13_2","first-page":"177","volume-title":"Flow Cytometry and Sorting","author":"Sweet RG","year":"1979"},{"key":"e_1_2_1_14_2","first-page":"644","article-title":"Characterization and enrichment of murine hemopoietic stem cells by fluorescence\u2010activated cell sorting","volume":"9","author":"Visser JWM","year":"1981","journal-title":"Exp Hematol"},{"key":"e_1_2_1_15_2","first-page":"95","volume-title":"Immunofluorescence Technology; Selected Theoretical and Clinical Aspects","author":"Visser JWM","year":"1982"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1986.tb03576.x"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990090208","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990090208","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T18:07:43Z","timestamp":1697911663000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990090208"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,3]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1988,3]]}},"alternative-id":["10.1002\/cyto.990090208"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990090208","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,3]]}}}