{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:00:25Z","timestamp":1762160425439},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":5545,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Syncytium formation between HUT\u201078 cells persistently infected with human immunodeficiency virus type 1 (HIV\u20101) and uninfected CD4\u2010bearing MOLT\u20104 or CEM cells results in a rapid destruction of the MOLT\u20104 or CEM cells. This syncytium formation is due to the interaction between the gp120 glycoprotein expressed by the persistently HIV\u20101\u2010in\u2010fected HUT\u201078 cells and the CD4 receptor present on MOLT\u20104 or CEM cells. A flow cytometric method has been applied to separate the infected (HUT\u201078) from the uninfected (MOLT\u20104, CEM) cell populations. This method is based on a modified DNA staining protocol which clearly shows the differences in DNA content between HUT\u201078 cells, on the one hand, and MOLT\u20104 or CEM cells, on the other hand. Using this flow cytometric method we have demonstrated that those compounds (i.e., sulfated polysaccharides, aurintricarboxylic acid) that interact with gp120 (of the HIV\u2010infected cells) or CD4 (of the uninfected cells) suppress syncytium formation and concomitant destruction of the CD4<jats:sup>+<\/jats:sup> cells.<\/jats:p>","DOI":"10.1002\/cyto.990110611","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:07:12Z","timestamp":1123517232000},"page":"736-743","source":"Crossref","is-referenced-by-count":17,"title":["Flow cytometric method to monitor the destruction of CD4<sup>+<\/sup> cells following their fusion with HIV\u2010infected cells"],"prefix":"10.1002","volume":"11","author":[{"given":"Dominique","family":"Schols","sequence":"first","affiliation":[]},{"given":"Rudi","family":"Pauwels","sequence":"additional","affiliation":[]},{"given":"Jan","family":"Desmyter","sequence":"additional","affiliation":[]},{"given":"Erik","family":"De Clercq","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.16.6132"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.34.1.134"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","unstructured":"BabaM. ScholsD. PauwelsR. NakashimaH. De ClercqE.: Sulfated polysaccharides as potent inhibitors of HIV\u2010induced syncytium formation: A new strategy towards AIDS chemotherapy.J Acquir Immun Defic Syndr(in press) 1990.","DOI":"10.1097\/00126334-199005000-00005"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(86)90877-6"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6189183"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/312763a0"},{"key":"e_1_2_1_8_2","first-page":"217","article-title":"Replication and pathogenesis of the AIDS virus","volume":"1","author":"Haseltine W. B.","year":"1988","journal-title":"J Acquir Immun Defic Syndr"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6328660"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3010463"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/323725a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3001934"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2452480"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.26.2.231-235.1988"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1128\/jcm.26.6.1229-1232.1988","article-title":"Rapid screening method with a cell multisizer for inhibitors of human immunodeficiency virus\u2010induced cell fusion in vitro","volume":"26","author":"Nakashima H.","year":"1988","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-3542(89)90033-8"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(88)90134-6"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6200935"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2430333"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(88)90149-4"},{"key":"e_1_2_1_21_2","first-page":"10","article-title":"Flow cytometric method to demonstrate whether anti\u2010HIV\u20101 agents inhibit virion binding to T4+ cells","volume":"2","author":"Schols D.","year":"1989","journal-title":"J Acquir Immun Defic Syndr"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.9.3322"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-70-9-2397"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","unstructured":"ScholsD. 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De ClercqE.: Dextran sulfate and other polyanionic anti\u2010HIV compounds specifically interact with the viral gp120 glycoprotein expressed by T\u2010cells persistently infected with HIV\u20101.Virology(in press) 1990.","DOI":"10.1016\/0042-6822(90)90440-3"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/322470a0"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990110611","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990110611","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T13:44:40Z","timestamp":1695735880000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990110611"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/cyto.990110611"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990110611","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}