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Rydberg (Int J Radiat Biol 46:521\u2013527, 1984). The ratio of green fluorescence (doublestranded DNA) to red fluorescence (single\u2010stranded DNA) in single cells was taken as a measure of DNA strand breaks. CHO\u2010K1 and M3\u20101 cells irradiated with X\u2010rays show a dose dependent induction of DNA strand breaks. Incubation at 37\u00b0C after irradiation leads to repair of breaks. A repair halflife of about 10\u201311 min can be determined.<\/jats:p><jats:p>Cell cycle specific differences in the induction of DNA strand breaks or repair behavior are not detectable at the resolution achieved so far.<\/jats:p><jats:p>This new method offers two major advantages: the resolution of DNA damage and repair on the level of single cells and no need for labeling, thereby allowing for DNA damage and repair to be assessed in biopsy material from tumor patients.<\/jats:p>","DOI":"10.1002\/cyto.990130107","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T15:48:15Z","timestamp":1123516095000},"page":"31-38","source":"Crossref","is-referenced-by-count":8,"title":["Assessment of radiation\u2010induced DNA strand breaks and their repair in unlabeled cells"],"prefix":"10.1002","volume":"13","author":[{"given":"Maja Item","family":"Affentranger","sequence":"first","affiliation":[]},{"given":"Werner","family":"Burkart","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553007314550311"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553007414551511"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553008214551231"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/09553008014551691"},{"key":"e_1_2_1_6_2","first-page":"291","volume-title":"Flow Cytometry and Sorting","author":"Darzynkiewicz Z","year":"1990"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01211107"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(79)90624-2"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-8140(89)90143-6"},{"key":"e_1_2_1_10_2","unstructured":"Item AffentrangerM BurkartW:Differences in the distribution of DNA strand breaks in single cells induced by X\u2010rays or bleomycin. 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