{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:21:20Z","timestamp":1779913280321,"version":"3.53.1"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":4449,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diploid human fibroblasts (IMR\u201090 cells), grown to confluency and growth\u2010arrested by serum starvation, were irradiated with a variety of doses of UV light (0.025\u201340 J\/m<jats:sup>2<\/jats:sup>) or incubated with broad dose ranges of four direct\u2010acting mutagens [ethyl methanesulfonate (EMS), ICR\u2010170, methyl methanesulfonate (MMS), and 4\u2010nitroquinoline oxide (4\u2010NQO)] and pulsed with a thymidine analog, 5\u2010bromodeoxyuridine (BrdUrd) to detect evidence of DNA repair. These cells and appropriate controls were immunochemically stained and subjected to flow cytometric analysis to quantify BrdUrd incorporation into DNA and simultaneously measure cellular DNA content. Since the maximal quantity of BrdUrd incorporated with repairing cells is profoundly less than the amount incorporated during replicative synthesis and flow cytometric analysis collects information on a cell\u2010to\u2010cell basis, data collection using linear histograms succeeded both in revealing repairing cellular populations and eliminating replicative cells from the analysis. Technical modifications necessary to achieve stoichiometry with UV\u2010irradiated IMR\u201090 fibroblasts included a 48h repair (and pulse) period, followed by denaturing cellular DNA for 15 min at 90\u00b0C. The limit of detection was equal to or below the lowest dose investigated (0.025 J\/m<jats:sup>2<\/jats:sup>). DNA repair was also detected with cultures incubated with low doses of all chemicals and pulsed with BrdUrd for a 24 h period. The limits of detection were near or below 500 m\u0308M EMS, 5 m\u0308M MMS, 0.25 m\u0308M 4\u2010NQO, and 0.1 m\u0308M ICR\u2010170. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990140207","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:26:18Z","timestamp":1123518378000},"page":"154-167","source":"Crossref","is-referenced-by-count":36,"title":["Statistical confirmation that immunofluorescent detection of DNA repair in human fibroblasts by measurement of bromodeoxyuridine incorporation is stoichiometric and sensitive"],"prefix":"10.1002","volume":"14","author":[{"given":"Jules R.","family":"Selden","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Dolbeare","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James 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