{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T18:27:52Z","timestamp":1764700072968},"reference-count":21,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":4145,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1993,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Multiparameter flow cytometry and cell sorting were used to examine the process of apoptosis after activation of murine resting T cells with immobilized anti\u2010CD3. Activated T cells treated with Hoechst 33342 (HO\u201033342) and analyzed by flow cytometry showed two major cell populations of high and low fluorescence. These populations were sorted and the DNA extracted and subjected to electrophoresis. Electrophoresis of DNA extracted from T cells showing a low level of HO\u201033342 fluorescence (HO\u2010Low) resulted in a typical ladder pattern characteristic of internucleosomal DNA degradation associated with apoptosis, whereas the cellular DNA of the cells showing a high level of fluorescence (HO\u2010High) showed a narrow high molecular weight band. Multiparameter analysis further indicated that cells with HO\u2010High characteristics possessed corresponding high\u2010FSC\/low\u2010SSC properties, whereas HO\u2010Low cells formed a cluster of low\u2010FSC\/high\u2010SSC cells. Analysis of the DNA extracted from cells sorted on the basis of scatter properties alone confirmed that the low\u2010FSC\/high\u2010SSC population contained the apoptotic cells and that the high\u2010FSC\/low\u2010SSC population was comprised of viable cells. This methodology allowed us to determine the percentage of apoptotic cells following anti\u2010CD3 activation at various time points and to discriminate them from those in cell cycle. We could further quantitate the number of apoptotic versus viable CD4+ and CD8+ cells in the cell cycle. \u00a9 1993 Wiley\u2010Liss, Inc.<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#note1\" \/><jats:fn><jats:p>This article is a US Government work and, as such, is in the public domain in the United States of America.<\/jats:p><\/jats:fn>\n<\/jats:p>","DOI":"10.1002\/cyto.990140806","type":"journal-article","created":{"date-parts":[[2005,8,8]],"date-time":"2005-08-08T16:03:18Z","timestamp":1123516998000},"page":"883-890","source":"Crossref","is-referenced-by-count":34,"title":["Identification and quantitation of apoptotic cells following anti\u2010CD3 activation of murine G<sub>0<\/sub> T cells"],"prefix":"10.1002","volume":"14","author":[{"given":"Francis J.","family":"Chrest","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meredith A.","family":"Buchholz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Ho","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taeg\u2010Kyu","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert A.","family":"Nordin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.10.040192.001411"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990130802"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(92)90048-G"},{"key":"e_1_2_1_5_2","volume-title":"Morphological and biochemical assays of apoptosis","author":"Duke RC","year":"1991"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90503-5"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(92)90238-K"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2125368"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"17","DOI":"10.4049\/jimmunol.149.1.17","article-title":"Expression of the murine homologue of the cell cycle control protein p34cdc2 in T lymphocytes","volume":"149","author":"Kim YH","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/353858a0"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990130803"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/343642a0"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"1801","DOI":"10.4049\/jimmunol.143.6.1801","article-title":"Calcium\u2010dependent killing of immature thymocytes by stimulation via the CD3\/T cell receptor complex","volume":"143","author":"McConkey DJ","year":"1989","journal-title":"J Immunol"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/347286a0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"2096","DOI":"10.4049\/jimmunol.144.6.2096","article-title":"T cell receptor\u2010mediated DNA fragmentation and cell death in T cell hybridomas","volume":"144","author":"Odaka CH","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830210214"},{"key":"e_1_2_1_17_2","volume-title":"Practical Flow Cytometry","author":"Shapiro HM","year":"1988"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/337181a0"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(91)90396-W"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990130205"},{"key":"e_1_2_1_21_2","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.4049\/jimmunol.143.11.3461","article-title":"Activation\u2010driven T cell death. 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