{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T04:43:35Z","timestamp":1773377015411,"version":"3.50.1"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":3811,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Neutral lipids accumulate in cellular lipid droplets. These droplets vary remarkably in number and amount between cells. In the present studies, the variability in lipid content was quantified by comparing the coefficient of variation of fluorescence histograms of nile red lipid\u2010stained cells to the variability of cell size or cell protein distributions. This measure of lipid droplet variability persisted through a wide range of cell lipid content and averaged 4.4\u2010fold more variability than cell size and 2.6\u2010fold more variability than cell protein content. While looking for possible explanations for this variability, it was determined that cell to cell variability could not be explained by multiple clonal populations of cells or the confluence of the cell monolayer. Analysis of lipid variability using a more droplet\u2010specific fluorescent dye, bodipy, reduced variability by about 44%. Cell cycle analysis revealed that G<jats:sub>2<\/jats:sub> + M cells contained more lipid than S\u2010phase cells, which in turn contained more lipid than G<jats:sub>0<\/jats:sub> + G<jats:sub>1<\/jats:sub> cells, but that variability was equally large throughout the cell cycle. The cholesteryl ester hydrolase inhibitor, diethylumbelliferyl phosphate, inhibited hydrolysis of both cholesteryl esters and triglycerides. Lipid content of diethylumbelliferyl phosphate\u2010treated cells increased while the variability in lipid staining decreased by an average of 72%. Thus, the excess lipid fluorescence variability compared to cell size or protein fluorescence could in part be explained by variability in cellular hydrolysis of triglyceride and cholesteryl ester. Excess lipid fluorescence variability could be reduced by an average of 44% when a more lipid droplet\u2010specific stain was used instead of nile red. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990170207","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T20:20:50Z","timestamp":1120162850000},"page":"151-158","source":"Crossref","is-referenced-by-count":200,"title":["Factors underlying the variability of lipid droplet fluorescence in MA\u201010 leydig tumor cells"],"prefix":"10.1002","volume":"17","author":[{"given":"Peter M.","family":"Gocze","sequence":"first","affiliation":[]},{"given":"Dale A.","family":"Freeman","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,6,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-108-1-88"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.52.070183.001255"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(67)12127-7"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1987.tb11065.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"13061","DOI":"10.1016\/S0021-9258(18)45167-8","article-title":"Cyclic\u2010AMP mediated modification of cholesterol traffic in Leydig tumor cells","volume":"262","author":"Freeman DA","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(83)90083-8"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"14231","DOI":"10.1016\/S0021-9258(19)45370-2","article-title":"Studies on the source of cholesterol used for steroid biosynthesis in cultured Leydig tumor cells","volume":"257","author":"Freeman DA","year":"1982","journal-title":"J Biol Chem"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3109\/10520297509117071","article-title":"Evaluation of six fluorescent protein stains for use in flow microfluorometry","volume":"50","author":"Freeman DA","year":"1975","journal-title":"Stain Technol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"1139","DOI":"10.1016\/S0022-2275(20)40765-5","article-title":"Accumulation and mobilization of triglycerides and cholesteryl esters in Leydig tumor cells","volume":"33","author":"Freeman DA","year":"1992","journal-title":"J Lipid Res"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.131.6.2972"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.100.3.965"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1016\/S0022-2275(20)42105-4","article-title":"Inhibitors of neutral cholesteryl ester hydrolase","volume":"31","author":"Harrison EH","year":"1990","journal-title":"J Lipid Res"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9150(90)90109-V"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/13.9.423"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2710809"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-126-5-2267"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"691","DOI":"10.1016\/S0022-2275(20)38329-2","article-title":"Elevation of liver diacylglycerol and molecular species of diacylglycerol in rats fed a lipogenic diet","volume":"30","author":"Ontko JA","year":"1989","journal-title":"J Lipid Res"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.103.2.273"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198602203140806"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(82)90004-2"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990170207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990170207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T05:00:47Z","timestamp":1698123647000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990170207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1002\/cyto.990170207"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990170207","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}