{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T15:14:49Z","timestamp":1764688489843},"reference-count":8,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,8]],"date-time":"2005-03-08T00:00:00Z","timestamp":1110240000000},"content-version":"vor","delay-in-days":3719,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Comparative genomic hybridization (CGH) is a new technique by which genomic imbalances can be detected by combining in situ suppression hybridization of whole genomic DNA and image analysis. We have developed software for rapid, quantitative CGH image analysis by a modification and extension of the standard software used for routine karyotyping of G\u2010banded metaphase spreads in the Magiscan chromosome analysis system. The DAPI\u2010counterstained metaphase spread is karyotyped interactively. Corrections for image shifts between the DAPI, FITC, and TRITC images are done manually by moving the three images relative to each other. The fluorescence background is subtracted. A mean filter is applied to smooth the FITC and TRITC images before the fluorescence ratio between the individual FITC\u2010 and TRITC\u2010stained chromosomes is computed pixel by pixel inside the area of the chromosomes determined by the DAPI boundaries. Fluorescence intensity ratio profiles are generated, and peaks and valleys indicating possible gains and losses of test DNA are marked if they exceed ratios below 0.75 and above 1.25. By combining the analysis of several metaphase spreads, consistent findings of gains and losses in all or almost all spreads indicate chromosomal imbalance. Chromosomal imbalances are detected either by visual inspection of fluorescence ratio (FR) profiles or by a statistical approach that compares FR measurements of the individual case with measurements of normal chromosomes. The complete analysis of one metaphase can be carried out in approximately 10 minutes. \u00a9 1995 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.990190106","type":"journal-article","created":{"date-parts":[[2005,6,30]],"date-time":"2005-06-30T21:21:58Z","timestamp":1120166518000},"page":"42-50","source":"Crossref","is-referenced-by-count":32,"title":["Image analysis in comparative genomic hybridization"],"prefix":"10.1002","volume":"19","author":[{"given":"Claes","family":"Lundsteen","sequence":"first","affiliation":[]},{"given":"Jan","family":"Maahr","sequence":"additional","affiliation":[]},{"given":"Britta","family":"Christensen","sequence":"additional","affiliation":[]},{"given":"Thue","family":"Bryndorf","sequence":"additional","affiliation":[]},{"given":"Martin","family":"Bentz","sequence":"additional","affiliation":[]},{"given":"Peter","family":"Lichter","sequence":"additional","affiliation":[]},{"given":"Tommy","family":"Gerdes","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,6,23]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"BentzM D\u00f6hnerH HuckK Sch\u00fctzB GanserA JoosS du ManoirS LichterP: Comparative Genomic Hybridization in the investigation of myeloid leukemias.Genes Chromosom Cancer(in press)."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-74738-0_15"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00202475"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1359641"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990070102"},{"key":"e_1_2_1_7_2","first-page":"493","article-title":"Clinical performance of a system for semiautomated chromosome analysis","volume":"41","author":"Lundsteen C","year":"1987","journal-title":"Am J Hum Genet"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00202476"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/2.11.1907"}],"container-title":["Cytometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcyto.990190106","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cyto.990190106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T19:57:46Z","timestamp":1698350266000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cyto.990190106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1002\/cyto.990190106"],"URL":"https:\/\/doi.org\/10.1002\/cyto.990190106","archive":["Portico"],"relation":{},"ISSN":["0196-4763","1097-0320"],"issn-type":[{"value":"0196-4763","type":"print"},{"value":"1097-0320","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1]]}}}