{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:58:02Z","timestamp":1757703482967},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,12]],"date-time":"2006-07-12T00:00:00Z","timestamp":1152662400000},"content-version":"vor","delay-in-days":5185,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Diagnostic Cytopathology"],"published-print":{"date-parts":[[1992,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Acanthamoeba species infect humans occasionally and act as opportunistic pathogens in immunocompromised individuals. This study demonstrates the application of cytocentrifugation as an aid to identification of Acanthamoebae. In addition, certain staining procedures clearly optimized visualization of characteristic amoebic features. This was demonstrated by adding amoebae from laboratory cultures to bronchoalveolar lavage specimens. In preparations stained by the Papanicolaou, trichrome, or hematoxylin\u2010eosin (H&amp;E) procedures, the discrete deeper staining nucleolus was the most distinctive feature. The vacuolated cytoplasm also aided in the identification of amoebae. These features were less apparent and often distorted following staining of Acanthamoeba species with the Hema III and Giemsa procedures. \u00a9 1992 Wiley\u2010Liss, inc.<\/jats:p>","DOI":"10.1002\/dc.2840080309","type":"journal-article","created":{"date-parts":[[2007,2,22]],"date-time":"2007-02-22T02:08:29Z","timestamp":1172110109000},"page":"231-234","source":"Crossref","is-referenced-by-count":13,"title":["Identification of <i>acanthamoeba<\/i> in bronchoalveolar lavage specimens"],"prefix":"10.1002","volume":"8","author":[{"given":"Anthony L.","family":"Newsome","sequence":"first","affiliation":[]},{"given":"Faune T.","family":"Curtis","sequence":"additional","affiliation":[]},{"given":"Clyde G.","family":"Culbertson","sequence":"additional","affiliation":[]},{"given":"Stephen D.","family":"Allen","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2006,7,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.3.490"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MR.52.1.114-133.1988"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000398093"},{"key":"e_1_2_1_5_2","first-page":"51","article-title":"Naegleria and Acanthamoeba free\u2010living amebas pathogenic for man","volume":"10","author":"Sotelo\u2010Avila C","year":"1987","journal-title":"Perspect Pediatr Pathol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1986.01050210072028"},{"key":"e_1_2_1_7_2","first-page":"491","article-title":"Cytologic diagnosis of Acanthamoeba keratitis","volume":"32","author":"Karayianis SL","year":"1987","journal-title":"Acta Cytol (Praha)"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.127.3313.1506"},{"key":"e_1_2_1_9_2","first-page":"185","article-title":"Experimental infection of mice and monkeys by Acanthamoeba","volume":"35","author":"Culbertson CG","year":"1959","journal-title":"Am J Pathol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1128\/am.28.1.64-65.1974","article-title":"Hemin replaces serum as a growth requirement for Naegleria","volume":"28","author":"Band RN","year":"1974","journal-title":"Appl Microbiol"},{"key":"e_1_2_1_11_2","first-page":"97","article-title":"Isolation of limax amoebas from the nasal mucosa of man","volume":"20","author":"Cerva L","year":"1973","journal-title":"Folia Parasitol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM196711302772204"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/71.2.201"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.3109\/08910608809141538"},{"key":"e_1_2_1_15_2","first-page":"749","article-title":"Acquired immunodeficiency syndrome associated with Acanthamoeba infection and other opportunistic organisms","volume":"110","author":"Gonzalez MM","year":"1986","journal-title":"Arch Pathol Lab Med"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.1.130"},{"key":"e_1_2_1_17_2","first-page":"668","article-title":"Cerebrospinal fluid centrifuge analysis in primary amebic meningoencephalitis due to Naegleria fowleri","volume":"109","author":"Benson RL","year":"1985","journal-title":"Arch Pathol Lab Med"}],"container-title":["Diagnostic Cytopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdc.2840080309","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dc.2840080309","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T12:55:32Z","timestamp":1698065732000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dc.2840080309"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,5]]},"references-count":16,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,5]]}},"alternative-id":["10.1002\/dc.2840080309"],"URL":"https:\/\/doi.org\/10.1002\/dc.2840080309","archive":["Portico"],"relation":{},"ISSN":["8755-1039","1097-0339"],"issn-type":[{"value":"8755-1039","type":"print"},{"value":"1097-0339","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,5]]}}}