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Previously we have shown that the mechanisms which regulate the translational activity of the r\u2010protein mRNAs operate at the level of translational initiation and do not involve changes in polyadenylation or capping. By analysing the translational behavior of chimeric and mutant mRNAs in transformed cells, we have now been able to localize the determinants of translational activity of one of the r\u2010protein mRNAs to the 5\u2032\u2010untranslated region. Although this and other r\u2010protein mRNAs differ strikingly from the <jats:italic>Dictyostelium<\/jats:italic> consensus in the region of the initiator AUG codon, we find that improving the match to that consensus does not increase the translational activity of the message in developing cells. Current experiments are designed to determine whether translational regulation is mediated by strong interactions with specific inhibitors or by weak interactions with translational initiation factors.<\/jats:p>","DOI":"10.1002\/dvg.1020120117","type":"journal-article","created":{"date-parts":[[2005,5,25]],"date-time":"2005-05-25T22:28:52Z","timestamp":1117060132000},"page":"98-103","source":"Crossref","is-referenced-by-count":24,"title":["Sequence elements that affect mRNA translational activity in developing <i>Dictyostelium<\/i> cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Laura F.","family":"Steel","sequence":"first","affiliation":[]},{"given":"Allan","family":"Jacobson","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,2,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90208-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90126-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00529a011"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04651.x"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.5.598"},{"key":"e_1_2_1_7_1","first-page":"161","article-title":"Analysis of mRNA transcription in Dictyostelium discoideum, or slime mold messenger RNA: How to find it and what to do with it once you've got it","volume":"8","author":"Jacobson A","year":"1976","journal-title":"Methods Mol Biol"},{"key":"e_1_2_1_8_1","volume-title":"Molecular Biology of Dictyostelium development","author":"Kimmel AR","year":"1988"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(87)90418-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.108.2.229"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/251385a0"},{"key":"e_1_2_1_12_1","volume-title":"The Development of Dictyostelium discoideum","author":"Loomis WF","year":"1982"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.1.1.35"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.4.12.2890"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90278-3"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80087-4"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90636-4"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.19.7989"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.23.9679"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6603(08)60614-5"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.3.965"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.1020090421"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.24.10285"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.13.5393"}],"container-title":["Developmental Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.1020120117","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.1020120117","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T13:32:40Z","timestamp":1693488760000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.1020120117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1]]},"references-count":23,"journal-issue":{"issue":"1-2","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1002\/dvg.1020120117"],"URL":"https:\/\/doi.org\/10.1002\/dvg.1020120117","archive":["Portico"],"relation":{},"ISSN":["0192-253X","1520-6408"],"issn-type":[{"value":"0192-253X","type":"print"},{"value":"1520-6408","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,1]]}}}