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Genet."],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>Saccharomyces cerevisiae CDC3<\/jats:italic>, <jats:italic>CDC10<\/jats:italic>, <jats:italic>CDC11<\/jats:italic>, and <jats:italic>CDC12<\/jats:italic> genes encode a family of homologous proteins that are not closely related to other known proteins [Haarer BK, Ketcham SR, Ford SK, Ashcroft DJ, and Pringle JR (submitted)]. Temperature\u2010sensitive mutants defective in any of these four genes display essentially identical pleiotropic phenotypes that include abnormal cell\u2010wall deposition and bud growth, an inability to complete cytokinesis, and a failure to form the ring of 10 nm filaments that normally lies directly subjacent to the plasma membrane in the neck region of budding cells. We showed previously that the <jats:italic>CDC3<\/jats:italic> and <jats:italic>CDC12<\/jats:italic> gene products localize to the region of the mother\u2010bud neck and are probably constituents of the ring of 10 nm filaments. We now report the generation of polyclonal antibodies specific for the <jats:italic>CDC11<\/jats:italic> product (Cdc11p) and the use of these antibodies in immunofluorescence experiments with wild\u2010type and mutant cells. The results suggest that Cdc11p is also a constituent of the filament ring, and thus support the hypothesis that the <jats:italic>S. cerevisiae<\/jats:italic> 10 nm filaments represent a novel type of eukaryotic cytoskeletal element. Cdc11p and actin both localize to the budding site well in advance of bud emergence and at approximately the same time, and both proteins also remain localized at the old budding site for some time after cytokinesis. Cdc11p also localizes to regions of cell\u2010wall reorganization in mating cells and in cells responding to purified mating pheromone. Surprisingly, most preparations of affinity purified Cdc11p\u2010specific antibodies also stained the nuclear and cytoplasmic microtubules. Although this staining probably reflects the existence of an epitope shared by Cdc11p and some microtubule\u2010associated protein, the possibility that a fraction of the Cdc11 p is associated with the microtubules could not be eliminated.<\/jats:p>","DOI":"10.1002\/dvg.1020120405","type":"journal-article","created":{"date-parts":[[2005,5,25]],"date-time":"2005-05-25T22:27:43Z","timestamp":1117060063000},"page":"281-292","source":"Crossref","is-referenced-by-count":157,"title":["Cellular morphogenesis in the <i>Saccharomyces cerevisiae<\/i> cell cycle: Localization of the <i>CDC11<\/i> gene product and the timing of events at the budding site"],"prefix":"10.1002","volume":"12","author":[{"given":"Susan K.","family":"Ford","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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