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During macronuclear development in <jats:italic>Tetrahymena<\/jats:italic>, the chromosomes fragment into pieces ranging from 20 kb to 1,500 kb. <jats:italic>Tetrahymena<\/jats:italic> telomerase, a ribonucleoprotein, adds telomeric (TTGGGG)<jats:sub>n<\/jats:sub> repeats onto telomeres and onto the newly generated macronuclear DNA ends. We have investigated whether telomerase RNA levels increase during macronuclear development, since such an increase might be expected during chromosomal fragmentation. The steady\u2010state level of the telomerase RNA component was used to estimate the abundance of telomerase present in mating and nonmating <jats:italic>Tetrahymena.<\/jats:italic> Northern blot analysis revealed that in vegetatively growing <jats:italic>Tetrahymena<\/jats:italic>, there were 18,000\u201340,000 copies of telomerase RNA per cell. In mating cultures, the levels of RNA increased 2\u2010to 5\u2010fold at 9\u201315 h, and 1.5\u2010 to 3.5\u2010fold in starved nonmating cultures. This increase in telomerase RNA paralleled telomerase activity, which also increased slightly in mating and starved nonmating cells. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.1020130113","type":"journal-article","created":{"date-parts":[[2005,5,25]],"date-time":"2005-05-25T22:32:00Z","timestamp":1117060320000},"page":"80-86","source":"Crossref","is-referenced-by-count":19,"title":["<i>Tetrahymena<\/i> Telomerase RNA levels increase during macronuclear development"],"prefix":"10.1002","volume":"13","author":[{"given":"Ariel A.","family":"Avilion","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lea A.","family":"Harrington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol W.","family":"Greider","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.16.5817"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.23.7383"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1016\/B978-0-12-273975-0.50009-7","volume-title":"The Molecular Biology of Ciliated Protozoa","author":"Blackburn EH","year":"1986"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-273975-0.50005-X"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.6.3.900"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(86)90045-5"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(83)90418-9"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90170-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90576-9"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/337331a0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.71.2.383"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150110503"},{"key":"e_1_2_1_14_1","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Maniatis T","year":"1982"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(82)90172-0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/7.3.547"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-273975-0.50006-1"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1979.tb02724.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.12.5569"},{"key":"e_1_2_1_20_1","first-page":"715","volume-title":"Mobile DNA","author":"Yao M C","year":"1989"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/14.5.2109"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90077-C"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/344126a0"}],"container-title":["Developmental Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fdvg.1020130113","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/dvg.1020130113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T20:18:42Z","timestamp":1698092322000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/dvg.1020130113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1002\/dvg.1020130113"],"URL":"https:\/\/doi.org\/10.1002\/dvg.1020130113","archive":["Portico"],"relation":{},"ISSN":["0192-253X","1520-6408"],"issn-type":[{"value":"0192-253X","type":"print"},{"value":"1520-6408","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}