{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T02:21:10Z","timestamp":1789784470971,"version":"4.0.1"},"reference-count":91,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,2,6]],"date-time":"2005-02-06T00:00:00Z","timestamp":1107648000000},"content-version":"vor","delay-in-days":4419,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Dev. Genet."],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The stimulation of translation in starfish oocytes by the maturation hormone, 1\u2010methyladenine (1\u2010MA), requires the activation or mobilization of both initiation factors and mRNAs [Xu and Hille, Cell Regul. 1:1057, 1990]. We identify here the translational initiation complex, eIF\u20104F, and the guanine nucleotide exchange factor for eIF\u20102, eIF\u20102B, as the rate controlling components of protein synthesis in immature oocytes of the starfish, <jats:italic>Pisaster orchraceus.<\/jats:italic> Increased phosphorylation of eIF\u20104E, the cap binding subunit of the eIF\u20104F complex, is coincident with the initial increase in translational activity during maturation of these oocytes. Significantly, protein kinase C activity increased during oocyte maturation in parallel with the increase in eIF\u20104E phosphorylation and protein synthesis. An increase in the activities of cdc2 kinase and mitogen\u2010activated myelin basic protein kinase (MBP kinase) similarly coincide with the increase in eIF\u20104E phosphorylation. However, neither cdc2 kinase nor MBP kinase phosphorylates eIF\u20104E in vitro. Casein kinase II activity does not change during oocyte maturation, and therefore, cannot be responsible for the activation of translation. Treatment of oocytes with phorbol 12\u2010myristate 13\u2010acetate, an activator of protein kinase C, for 30 min prior to the addition of 1\u2010MA resulted in the inhibition of 1\u2010MA\u2010induced phosphorylation of eIF\u20104E, translational activation, and germinal vesicle breakdown. Therefore, protein kinase C may phosphorylate eIF\u20104E, after very early events of maturation. Another possibility is that eIF\u20104E is phosphorylated by an unknown kinase that is activated by the cascade of reactions stimulated by 1\u2010MA. In conclusion, our results suggest a role for the phosphorylation of eIF\u20104E in the activation of translation during maturation, similar to translational regulation during the stimulation of growth in mammalian cells. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/dvg.1020140604","type":"journal-article","created":{"date-parts":[[2005,5,25]],"date-time":"2005-05-25T22:39:03Z","timestamp":1117060743000},"page":"424-439","source":"Crossref","is-referenced-by-count":23,"title":["Maturation hormone induced an increase in the translational activity of starfish oocytes coincident with the phosphorylation of the mRNA cap binding protein, eIF\u20104E, and the activation of several kinases"],"prefix":"10.1002","volume":"14","author":[{"given":"Zhe","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaydev N.","family":"Dholakia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merrill B.","family":"Hille","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,6]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.3.998"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(92)90499-B"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(86)24027-6"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"11134","DOI":"10.1016\/S0021-9258(18)60935-4","article-title":"Involvement of the 24\u2010kDa capbinding protein in regulation of protein synthesis in mitosis","volume":"262","author":"Bonneau AM","year":"1987","journal-title":"J Biol Chem"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"4975","DOI":"10.1016\/S0021-9258(18)53491-8","article-title":"Novel phosphorylation of sites of eukaryotic initiation factor\u20104F and evidence that phosphorylation stabilizes interactions of the \u221225 and \u2212220 subunits","volume":"268","author":"Bu X","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(86)90155-8"},{"key":"e_1_2_1_8_1","first-page":"15180","article-title":"Definition of a consensus sequence for peptide substrate recognition by p44mpk, the meiosis\u2010activated myelin basic protein kinase","volume":"26","author":"Clark\u2010Lewis I","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90394-0"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.1.51"},{"key":"e_1_2_1_11_1","doi-asserted-by":"crossref","first-page":"3162","DOI":"10.1016\/S0021-9258(18)49968-1","article-title":"Epidermal growth factor or okadaic acid stimulates phosphorylation of eukaryotic initiation factor 4F","volume":"266","author":"Donaldson RW","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_12_1","first-page":"237","article-title":"Calcium\u2010mediated transduction of the hormonal message in meiosis reinitiation of starfish oocytes","volume":"135","author":"Doree M","year":"1981","journal-title":"Modulation following injection of cholera toxin and cAMP\u2010dependent protein kinase. 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