{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T12:21:32Z","timestamp":1779366092752,"version":"3.53.0"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,9,14]],"date-time":"2007-09-14T00:00:00Z","timestamp":1189728000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Genesis"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The Cre\/loxP system has been used extensively for conditional mutagenesis in mice. Reporters of Cre activity are important for defining the spatial and temporal extent of Cre\u2010mediated recombination. Here we describe\n                    <jats:italic>mT\/mG<\/jats:italic>\n                    , a double\u2010fluorescent Cre reporter mouse that expresses membrane\u2010targeted tandem dimer Tomato (mT) prior to Cre\u2010mediated excision and membrane\u2010targeted green fluorescent protein (mG) after excision. We show that reporter expression is nearly ubiquitous, allowing visualization of fluorescent markers in live and fixed samples of all tissues examined. We further demonstrate that mG labeling is Cre\u2010dependent, complementary to mT at single cell resolution, and distinguishable by fluorescence\u2010activated cell sorting. Both membrane\u2010targeted markers outline cell morphology, highlight membrane structures, and permit visualization of fine cellular processes. In addition to serving as a global Cre reporter, the\n                    <jats:italic>mT\/mG<\/jats:italic>\n                    mouse may also be used as a tool for lineage tracing, transplantation studies, and analysis of cell morphology in vivo. genesis 45:593\u2013605, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.\n                  <\/jats:p>","DOI":"10.1002\/dvg.20335","type":"journal-article","created":{"date-parts":[[2007,9,14]],"date-time":"2007-09-14T16:17:22Z","timestamp":1189786642000},"page":"593-605","source":"Crossref","is-referenced-by-count":3402,"title":["A global double\u2010fluorescent Cre reporter mouse"],"prefix":"10.1002","volume":"45","author":[{"given":"Mandar Deepak","family":"Muzumdar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bosiljka","family":"Tasic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunari","family":"Miyamichi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,9,14]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.22.11984"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0102-83"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8303295"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1046"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.07.013"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8016642"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/gene.10021"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2002.0597"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.2000.9732"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90662-X"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.749203"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1999.9209"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-5-20"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200636745"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.1.324"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/13657"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606491104"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-968X(200002)26:2<99::AID-GENE1>3.0.CO;2-B"},{"key":"e_1_2_1_20_1","first-page":"95","article-title":"Creation 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