{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:05:53Z","timestamp":1762067153526},"reference-count":68,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":12038,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1972,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ultrastructural distribution of membrane\u2010bound immunoglobulin (Ig) on mouse spleen lymphocytes has been studied by labeling the surface Ig with anti\u2010mouse Ig antibody conjugated to ferritin (FT). The distribution is temperature\u2010dependent. At 0 \u00b0C \u2013 4 \u00b0C FT appears distributed in small clusters over the entire surface, whereas at 20 \u00b0C \u2013 22 \u00b0C, in the great majority of the labeled cells, it is concentrated over one pole. This pole corresponds to that part of the cell which contains the Golgi complex and most of the cellular organelles, and which forms the uropod in moving lymphocytes. This polar redistribution is accompanied by endocytosis of part of the labeled membrane.<\/jats:p><jats:p>The redistribution of label occurring at room temperature indicates that labeled and unlabeled components of the plasma membrane can segregate from each other and suggests that plasma membrane is fluid at room temperature. The diffuse distribution found at 0 \u00b0C \u2013 4 \u00b0C is probably close (although not identical) to the distribution <jats:italic>in vivo<\/jats:italic> before labeling, while the polar distribution at higher temperature is probably induced by the cross\u2010linking of the surface Ig molecules by the antibody\u2010FT conjugate and by a metabolically\u2010dependent flow of the aggregates towards the \u201ctail\u201d of the lymphocyte. The mechanism involved is considered to be the same mechanism responsible for membrane flow during cell movement. A detailed model of the structure and rheological properties of the lymphocyte membrane is proposed, which can explain the present results and those of others concerning the behavior of surface components of lymphocytes and other cells.<\/jats:p>","DOI":"10.1002\/eji.1830020611","type":"journal-article","created":{"date-parts":[[2007,2,27]],"date-time":"2007-02-27T22:06:46Z","timestamp":1172614006000},"page":"523-535","source":"Crossref","is-referenced-by-count":162,"title":["Distribution of immunoglobulin on the surface of mouse lymphoid cells as determined by immunoferritin electron microscopy, Antibody\u2010induced, temperature\u2010dependent redistribution and its implications for membrane structure"],"prefix":"10.1002","volume":"2","author":[{"given":"S.","family":"De Petris","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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