{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,1]],"date-time":"2024-07-01T05:15:09Z","timestamp":1719810909007},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":11338,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1974,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Using a <jats:sup>51<\/jats:sup>Cr release cytotoxicity assay, the cytotoxic effector phase of <jats:italic>in vitro<\/jats:italic> activated mouse T lymphocytes (killer cells) against <jats:sup>51<\/jats:sup>Cr\u2010labeled target cells has been investigated. It is shown that within 5\u201310 minutes of contact between killer cells and target cells, the target cells are already committed to lysis, therefore, antigen recognition and \u201clethal hit\u201d must have taken place within this period of time. In contrast, target cell lysis (cytolysis phase) requires up to 3\u20134 h in order to be completed; it occurs independently of killer cells and it is highly temperature dependent.<\/jats:p><jats:p>The killer cell\u2010dependent phase (antigen\u2010recognition and \u201clethal hit\u201d) is dissociated into two consecutive steps. The first step represents a specific and reversible event which takes place both at 4 \u00b0C and 37 \u00b0C. In contrast, the second step is found to be temperature dependent, but irreversible, and commits the target cells to lysis. It is concluded that the antigen recognition phase takes place during the first step and that the second step represents the \u201clethal hit\u201d phase.<\/jats:p>","DOI":"10.1002\/eji.1830041108","type":"journal-article","created":{"date-parts":[[2007,2,27]],"date-time":"2007-02-27T23:39:21Z","timestamp":1172619561000},"page":"745-750","source":"Crossref","is-referenced-by-count":46,"title":["T cell\u2010mediated cytotoxicity: Discrimination between antigen recognition, lethal hit and cytolysis phase"],"prefix":"10.1002","volume":"4","author":[{"given":"H.","family":"Wagner","sequence":"first","affiliation":[]},{"given":"M.","family":"R\u00f6llinghoff","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60308-9"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830010620"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/229566a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.135.4.890"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.134.2.553"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(72)90004-4"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.136.6.1594"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.1.177"},{"key":"e_1_2_1_10_2","first-page":"779","volume":"33","author":"Miller R. 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Immunol."},{"key":"e_1_2_1_19_2","first-page":"71","volume":"17","author":"Berke G.","year":"1973","journal-title":"Transplant. Rev."},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830020418"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.6.1620"},{"key":"e_1_2_1_22_2","volume-title":"Immunological Parameters of Host\u2010Tumor Relationship","author":"Berke G.","year":"1974"},{"key":"e_1_2_1_23_2","volume-title":"Immunological Approaches to Problems in Microbiology","author":"Mayer M. 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