{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T16:14:37Z","timestamp":1699805677282},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":10881,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1976,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Papain\u2010solubilized HLA antigens giving only two bands of 34 000 mol.wt. and 11 000 mol.wt. by sodium dodecyl sulfate (SDS) gel electrophoresis and isolated from the cultured human lymphoblastoid cell line RPMI 4265, have been used to prepare antisera in rabbits. Antisera were raised against soluble products of the A (or 1st) series of HLA, bearing the determinant HLA\u2010A2 (A2 substance), or against a mixture of products of the B (or 2nd) series of HLA, bearing the determinants HLA\u2010B7 and HLA\u2010B 12 (B7\u201012 substances). Rabbit antisera to A2 substance reacted primarily with A2 substance on Ouchterlony analysis, showing an apparent spur of cross\u2010reactivity with B7\u201012 substances. Rabbit antisera to B7\u201012 substances reacted primarily with B7\u201012 substances, giving a spur of cross\u2010reactivity with the A2 material. Neither antiserum precipitated \u03b2\u2010<jats:sub>2<\/jats:sub>microglobulin. Both types of sera reacted with membrane molecules of 43 000 mol.wt. and 11 000 mol.wt. by immune precipitation and gel electrophoresis in SDS of detergent\u2010solubilized radiolabeled membranes from cultured cell lines, as predicted for sera directed towards HLA antigens. F(ab\u2032)<jats:sub>2<\/jats:sub> fragments of the antibodies blocked the complement\u2010mediated cytotoxicity of all HLA alloantisera tested for human peripheral blood lymphocytes. After absorption with B7\u201012 substances, F(ab\u2032)<jats:sub>2<\/jats:sub> fragments of antisera to A2 substance only blocked the cytotoxicity of HLA alloantisera to A series specificities. After absorption with A2 substance, F(ab\u2032)<jats:sub>2<\/jats:sub> fragments of rabbit antisera to B7\u201012 substances only blocked the cytotoxicity of HLA alloantisera to B series specificities. The results prove the existence of shared antigenic determinants between all members of the same series. These findings support the genetic evidence that A series HLA antigens are allelic products of a single locus, while B series HLA antigens are allelic products of a separate locus, by establishing some invariance of structure, presumably amino acid sequence, between members of the same series. The apparent cross\u2010reactivity between A2 substance and B7\u201012 substances, and the ability of the unabsorbed F(ab\u2032)<jats:sub>2<\/jats:sub>, preparations to block the cytotoxicity of all HLA alloantisera, suggests that some determinants are common to both HLA loci. This may be considered to support the hypothesis that the two loci arose by gene duplication.<\/jats:p>","DOI":"10.1002\/eji.1830060203","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T03:03:34Z","timestamp":1172631814000},"page":"82-88","source":"Crossref","is-referenced-by-count":22,"title":["HLA antigens: rabbit antisera reacting with all A series or all B series specificities"],"prefix":"10.1002","volume":"6","author":[{"given":"P.","family":"Cresswell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet L.","family":"Ayres","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"415","volume":"5","author":"Eijsvoogel V. P.","year":"1973","journal-title":"Transplant. Proc."},{"key":"e_1_2_1_3_2","first-page":"27","volume":"6","author":"Amos D. 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