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Treatment of thymocyte membranes with urea\u2010Nonidet\u2010P40 (NP40) proved to be the most valuable method, since nearly 100% of the antigen was solubilized essentially without loss of the antigenicity. By gel filtration in urea\u2010NP4O the molecular weight was estimated to be 35 000 and the Stokes radius in sodium deoxycholate 31.8 \u00c5. In gel filtration studies a homogenous peak was obtained, whereas isoelectric focusing yielded two peaks (isoelectric points pH 4.5\u20135.0 and pH 5.4). The three determinants of this antigenic system were inseparable in gel filtration and isoelectric focusing, supporting the view put forward on the basis of serological studies that they are part of the same molecule.<\/jats:p><jats:p>Extraction of murine thymocyte membranes with organic solvents yielded a considerable loss of activity of the thymocytes\u2010brain antigen. There was some residual activity in the protein fraction, but none in the organic phase. Correspondingly, the highly purified ganglioside G<jats:sub>Gtet<\/jats:sub> 2b proved unable to absorb any rabbit anti\u2010 CBA brain serum. The reduced antigenic activity of the delipidated protein fraction could be restored by addition of lipids, as well as the nonionic detergent NP40. These observations suggest that interaction of protein and lipid is necessary for the antigenicity of the thymocyte\u2010brain antigen.<\/jats:p>","DOI":"10.1002\/eji.1830060506","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T03:31:23Z","timestamp":1172633483000},"page":"333-340","source":"Crossref","is-referenced-by-count":20,"title":["Solubilization and molecular characterization of membrane\u2010bound antigens shared by thymocytes and brain"],"prefix":"10.1002","volume":"6","author":[{"given":"R.","family":"Arndt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosemarie","family":"Stark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelika","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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