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These antisera were shown to be reactive in complement and antibody\u2010dependent cell\u2010mediated cytotoxicity tests as well as in immunofluorescence, with 30 % of mouse spleen cells, 80 % of lymph node cells and 100 % of thymocytes or various T lymphoblastoid cell lines labeled.<\/jats:p><jats:p>No reaction could be detected with bone marrow cells or non\u2010T cell lines. Both types of reagents bound to the same spleen lymphocyte subpopulations and competed for the same antigenic sites as anti\u2010Thy\u20101.2 alloantiserum. 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