{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:27:14Z","timestamp":1759332434330},"reference-count":22,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":10607,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1976,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Murine T cells were sensitized <jats:italic>in vitro<\/jats:italic> towards fluorescein isothiocyanate (FITC)\u2010conjugated syngeneic spleen cells. The lytic activity of the effector cells generated was restricted to FITC\u2010conjugated, H\u20102\u2010compatible target cells. 2,4,6\u2010Trinitrophenyl (TNP)\u2010conjugated syngeneic targets were not lysed.<\/jats:p><jats:p>A method is described for the <jats:italic>in vivo<\/jats:italic> sensitization of murine T cells towards TNP (or FITC)\u2010conjugated syngeneic spleen cells. The procedure is based on the observation that a local graft of hapten\u2010conjugated syngeneic spleen cells results in the development of sensitized prekiller T cells within the draining lymph node (LN). By mere <jats:italic>in vitro<\/jats:italic> incubation of such LN cells immunological specific anti TNP (or anti\u2010FITC)\u2010reactive cytotoxic T lymphocytes (CTL) are generated. The <jats:italic>in vitro<\/jats:italic> differentiation of CTL requires 48\u201072 h; it is dependent on cell proliferation and is abolished by treatment of the LN cells with mitomycin C. Thus there is a separation between the sensitization of CTL precursors (which effectively takes place <jats:italic>in vivo<\/jats:italic>) and the phase of cell differentiation into CTL (which effectively takes place only <jats:italic>in vitro<\/jats:italic>). Similar to <jats:italic>in vitro<\/jats:italic>\u2010sensitized CTL, the specificity of <jats:italic>in vivo<\/jats:italic>\u2010sensitized CTL is dictated by the hapten used for the induction of the immune response and is restricted to hapten\u2010conjugated syngeneic target cells.<\/jats:p>","DOI":"10.1002\/eji.1830061109","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T02:36:43Z","timestamp":1172630203000},"page":"799-805","source":"Crossref","is-referenced-by-count":44,"title":["<i>In vivo<\/i> sensitization of T cells to hapten\u2010conjugated syngeneic structures of major histocompatibility complex. I. Effect of <i>in vitro<\/i> culture upon generation of cytotoxic T lymphocytes"],"prefix":"10.1002","volume":"6","author":[{"given":"A.","family":"Starzinski\u2010Powitz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Pfizenmaier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"R\u00f6llinghoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Wagner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830040802"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.142.2.403"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.2.625"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.143.1.127"},{"key":"e_1_2_1_6_2","first-page":"222","volume":"29","author":"Shearer G. 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