{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,1,13]],"date-time":"2024-01-13T22:05:05Z","timestamp":1705183505959},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":10218,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1977,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thrinitropheny1 (TNP)\u2010specific primary IgM plaque\u2010forming cell responses were generated in vitro by unprimed spleen cells to the soluble antigens TNP\u2010keyhole limpet hemocyanin, trinitrophenylated poly\u2010L(Tyr\u2010Glu)\u2010poly\u2010D,L\u2010Ala\u2013poly\u2010L\u2010Lys[T,G)\u2010A\u2013L] and poly\u2010L(His,Glu)\u2010poly\u2010D,L\u2010Ala\u2013poly\u2010L\u2010Lys[(H,G)\u2010A\u2013L].The T cell dependence of these primary lgm responses was esablished by (a) abrogation of responses after pretreatment of cells with a T cell\u2010specific rabbit anti\u2010mouse brain serum (RaMB)and C\u2010treated spleen cells (B cells); and (c) absence of significant responses by spleen cells from congenic nude mice. A requirement for macrophages in these same antibody responses was also demonstrated:(a) responses were abrogated or strongly diminished in spleen cell populations depleted of macrophages by passage over Sephadex G\u201010 columns; and (b) these responses were fully reconstituted by the addition of small numbers of adherent, radioresistant, anti\u2010Thy\u20101.2\u2010treated spleen cells (macrophages) to the Sephadex G\u201010\u2010depleted populations. In addition, H\u20102\u2010linked Ir gene control has been demonstrated for the primary IgM responses to TNP conjugates of (T,G)\u2010A\u2013L and (H,G)\u2010A\u2013L. A system has thus been characterized which permits in vitro analysis of the cellular and genetic requirements involved in primary IgM responses to soluble antigens, including those under Ir gene control.<\/jats:p>","DOI":"10.1002\/eji.1830071214","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T04:26:39Z","timestamp":1172636799000},"page":"892-897","source":"Crossref","is-referenced-by-count":25,"title":["Cellular and genetic control of antibody responses in vitro 1.cellular requirements for the generation of genetically controlled primary IgM responses to soluble antigens"],"prefix":"10.1002","volume":"7","author":[{"given":"R.J.","family":"Hodes","sequence":"first","affiliation":[]},{"given":"A.","family":"Singer","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.144.5.1254"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.144.2.371"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.138.5.1107"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.4049\/jimmunol.118.4.1306","volume":"118","author":"Eardley D.D.","year":"1977","journal-title":"J. Immunol."},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/newbio245285a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-132-34264"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.140.6.1646"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(74)90108-2"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.126.3.423"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.140.3565.405"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.135.5.1049"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/260324a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.138.5.1121"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830071214","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830071214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T17:00:25Z","timestamp":1698944425000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830071214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,12]]},"references-count":13,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1977,12]]}},"alternative-id":["10.1002\/eji.1830071214"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830071214","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,12]]}}}