{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:28:09Z","timestamp":1759332489503},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,11]],"date-time":"2005-12-11T00:00:00Z","timestamp":1134259200000},"content-version":"vor","delay-in-days":10147,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1978,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A previous study (<jats:italic>Eur. J. Immunol.<\/jats:italic> 1977. <jats:italic>7<\/jats:italic>: 714) has shown that mice injected intravenously (i.v.) with 1 \u00d7 10<jats:sup>9<\/jats:sup> sheep red blood cells (SRBC) produce cells which suppress delayed\u2010type hypersensitivity (DTH). These suppressor cells are \u0398\u2010positive, antigen\u2010specific and act via a soluble factor which does not bear immunoglobulin determinants (<jats:italic>Eur. J. Immunol.<\/jats:italic> 1978. <jats:italic>8<\/jats:italic>: 168). The present paper demonstrates that these suppressor cells are inhibitable by cyclophosphamide (CY). Mice injected with graded amounts of CY two days prior to SRBC injection, showed maximum augmentation of DTH at 200 mg\/kg body weight, a dose which completely suppressed the appearance of splenic plaque\u2010forming cells (PFC) to SRBC. In contrast, lower doses of CY enhanced both DTH and PFC responses. Time course studies showed that CY inhibited the precursors of suppressor cells and had little or no effect on suppressor cells which have already encountered antigens. This was further confirmed by passive transfer studies which showed that suppressor cells were inhibited if CY was administered at the same time or 2 days before SRBC injection, but were not affected if CY was given after antigen stimulation. Direct evidence for the effect of CY on suppressor cells was obtained by cell fractionation with a Ficoll density gradient. The denser suppressor cell population was absent from the spleens of mice treated with 200 mg\/kg of CY 2 days before i.v. injection with 1 \u00d7 10<jats:sup>9<\/jats:sup> SRBC.<\/jats:p>","DOI":"10.1002\/eji.1830080306","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T09:36:39Z","timestamp":1172655399000},"page":"172-176","source":"Crossref","is-referenced-by-count":72,"title":["Regulation of delayed\u2010type hypersensitivity. III. Effect of cyclophosphamide on the suppressor cells for delayed\u2010type hypersensitivity to sheep erythrocytes in mice"],"prefix":"10.1002","volume":"8","author":[{"given":"H. K.","family":"Gill","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. Y.","family":"Liew","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,11]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"493","volume":"23","author":"Turk J. L.","year":"1972","journal-title":"Immunology"},{"key":"e_1_2_1_3_2","first-page":"701","volume":"24","author":"Turk J. L.","year":"1973","journal-title":"Immunology"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.139.6.1529"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.141.3.697"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830071013"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.7.2486"},{"key":"e_1_2_1_8_2","first-page":"1027","volume":"32","author":"Kerkhaert J. A. M.","year":"1977","journal-title":"Immunology"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.137.2.343"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.143.5.1199"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/251550a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"333","DOI":"10.4049\/jimmunol.112.1.333","volume":"112","author":"Moorhead J. W.","year":"1974","journal-title":"J. Immunol."},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/249654a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830080305"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830061003"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-8749(75)80021-9"},{"key":"e_1_2_1_17_2","first-page":"899","volume":"32","author":"Balow J. 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