{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:03:09Z","timestamp":1762160589026},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":9116,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1980,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Analysis of reactivity of monoclonal anti\u2010Ia<jats:sup>k<\/jats:sup> alloantibodies, obtained by fusion between NS 1 myeloma and spleen cells from alloimmune A.TH mice, permitted the identification of 9 distinct determinants of the I<jats:sup>k<\/jats:sup> gene products. Competitive binding experiments indicated that 2 private epitopes (defined by H 8\u2013109.13 and H 8\u2013138.4 antibodies) of the I\u2010A<jats:sup>k<\/jats:sup> product could be separated, thereby apparently splitting the Ia.2 specificity. A public determinant of the I\u2010A<jats:sup>k<\/jats:sup> molecule (identified by H 8\u201315.9 antibody) was found expressed not only on the 1\u2010A products of the H\u20102<jats:sup>b<\/jats:sup>, H\u20102<jats:sup>d<\/jats:sup>, H\u20102<jats:sup>ja<\/jats:sup>, H\u20102<jats:sup>P<\/jats:sup> and H\u20102<jats:sup>q<\/jats:sup> murine haplotypes, but also on human HLA\u2010DR antigens. Four determinants of the I\u2010E\/C<jats:sup>k<\/jats:sup> antigen (defined by H 7\u20138.26, H 10\u201381.10, H 10\u201393.2 and H 8\u201386.2 antibodies) had a strain distribution analogous to the Ia.7 specificity. However, competitive binding experiments, and the cross\u2010reactivity pattern with la\u2010like antigens from other species (<jats:italic>e.g.<\/jats:italic> human HLA\u2010DR antigens) indicated that these antibodies detected distinct determinants on the I\u2010E\/C<jats:sup>k<\/jats:sup> molecule, thereby subdividing the broad Ia.7 specificity. Two other determinants (defined by H9\u201314.8 and H9\u201315.4 antibodies) had a strain distribution that did not permit a precise assignment to a given Ia antigen, even though preliminary data suggested that they could detect separate determinants on the I\u2010E\/C<jats:sup>k<\/jats:sup> product. All these monoclonal antibodies identified membrane antigens with the expected Ia tissue distribution pattern, and most of them could precipitate a molecule containing two chains of 28 kD and 35 kD, from mouse spleen cell lysates.<\/jats:p>","DOI":"10.1002\/eji.1830101211","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T12:20:17Z","timestamp":1172665217000},"page":"950-957","source":"Crossref","is-referenced-by-count":81,"title":["Distinct epitopes on I<sup>k<\/sup> gene products identified by monoclonal antibodies"],"prefix":"10.1002","volume":"10","author":[{"given":"Michel","family":"Pierres","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7ois M.","family":"Kourilsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Paul","family":"Rebouah","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magali","family":"Dosseto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle","family":"Caillol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-48287-8"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1978.tb00385.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60208-4"},{"key":"e_1_2_1_5_2","first-page":"236","volume":"30","author":"Cullen S. 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