{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T22:14:52Z","timestamp":1759702492148},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":9085,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1981,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Lyt phenotype of T cells at different stages of response to mutant H\u20102K antigens was determined by immunofluorescence using monoclonal rat anti\u2010Lyt antibodies. Previous observations indicated a differential expression of the two allelic forms of Lyt\u20101 antigen on these cells. Since the rat antibodies recognize nonpolymorphic framework determinants of Lyt antigens, in our approach the expression of both Lyt\u20101 alleles was analyzed with the same antibody. It was found that cells reacting to three different H\u20102K mutants have the Lyt\u20101,2 phenotype, regardless of the Lyt\u20101 allele carried by the responder strain. The Lyt phenotype of responder cells remained unchanged after priming <jats:italic>in vivo<\/jats:italic>. However, cells recovered from cultures after secondary stimulation <jats:italic>in vitro<\/jats:italic> were mainly Lyt\u20102, with few Lyt\u20101,2 and virtually no Lyt\u20101 cells present. This change of Lyt phenotype ran in parallel with the loss of proliferative capacity to the priming antigen, but cytolytic activity of the cells remained unimpaired. Long\u2010term proliferation of T cells induced against mutant H\u20102K antigens could only be maintained in the presence of a T cell growth factor. Cultures with growth factor contained almost exclusively Lyt\u20102 cells and exerted strong cytolytic activity. These results demonstrate that the Lyt differentiation pathway of anti\u2010mutant T cells is from Lyt\u20101,2 to Lyt\u20102. Furthermore, the data suggest that no helper cells are induced in response to mutant H\u20102K antigens. A model which incorporates these findings into current concepts of T cell differentiation is discussed.<\/jats:p>","DOI":"10.1002\/eji.1830110302","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T13:44:08Z","timestamp":1172670248000},"page":"167-172","source":"Crossref","is-referenced-by-count":17,"title":["Terminal differentiation of T cells specific for mutant H\u20102K antigens. Conversion of Lyt\u20101,2 cells into Lyt\u20102 but not Lyt\u20101 cells, <i>in vitro<\/i>"],"prefix":"10.1002","volume":"11","author":[{"given":"Zoltan A.","family":"Nagy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piotr","family":"Kusnierczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/253219a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.141.6.1376"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.141.6.1390"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1101\/SQB.1977.041.01.006"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.148.5.1414"},{"key":"e_1_2_1_7_2","first-page":"96","volume":"156","author":"Simon M. 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