{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T02:07:05Z","timestamp":1782439625769,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":8746,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapidly\u2010growing, HAT\u2010sensitive cell line LICR\u2010LON\u2010HMy2 has been derived from the ARH\u201077 human plasma cell leukemia\u2010derived line. It lacks the enzyme hypoxanthine phosphoribosyltransferase (EC 2.4.2.8). Hybrids have been reproducibly made for more than a year and in independent laboratories with lymphocytes from tonsils, lymph nodes and peripheral blood and tonsil lymphocytes cultured with antigens. The parent line and hybrids are very robust in culture and double in 20\u201030 h. Hybrids clone easily and have stable karyotypes, most with modal numbers in the sixties. Stable Ig secretion patterns have been observed over 20\u201030 passages, and after cloning. It was estimated that about half the hybrids produce new immunoglobulin chains in addition to the parent cell line's IgG<jats:sub>1<\/jats:sub> (\u00d7 light chain). High, but not limiting, density hybridoma cultures (approximately 5 \u00d7 10<jats:sup>5<\/jats:sup> cells\/ml) typically produce 0.25\u20102 \u03bcg\/ml immunoglobulin per day, but some hybrids produce more. A high\u2010secreting variant of LICR\u2010LON\u2010HMy2 has been derived. The LICR\u2010LON\u2010HMy2 line is human and is distinct from the HAT\u2010sensitive human B cell\u2010lines SKO\u2010007 and GM 1500\u20106TG A1 2. It is available for distribution.<\/jats:p>","DOI":"10.1002\/eji.1830120804","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T19:00:55Z","timestamp":1172689255000},"page":"641-648","source":"Crossref","is-referenced-by-count":155,"title":["A human\u2010human hybridoma system based on a fast\u2010growing mutant of the ARH\u201077 plasma cell leukemia\u2010derived line"],"prefix":"10.1002","volume":"12","author":[{"given":"Paul A. W.","family":"Edwards","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clare M.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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