{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:37:20Z","timestamp":1779345440810,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":8743,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Monoclonal antibodies (mAb) derived from BALB\/c mice immunized with human anti\u2010HLA\u2010A2 cloned cytotoxic T lymphocytes (CTL) were screened for their ability to block, in the absence of complement, the cytolytic activity of the immunizing CTL clone. Eight cytolysis\u2010inhibiting mAb have been derived. One of these was directed against a monomorphic determinant expressed on HLA\u2010class I molecules and thus probably inhibited cytolysis via a target cell antigen\u2010masking effect. The 7 other mAb recognized \u201cCTL function\u2010associated structures\u201d and did not have to interfere with target cell antigens in order to inhibit cytolysis. F(ab')<jats:sub>2<\/jats:sub> and Fab fragments of these 7 mAb were also inhibitory. Competitive inhibition of binding and preliminary biochemical analysis suggested that these 7 mAb defined on cloned CTL various epitopes of a structure of 30 kDa disulfide\u2010bonded into several multimeric forms when analyzed without reduction. The inhibitory effect of these 7 mAb has been investigated on a series of short\u2010term (1 month) and long\u2010term (&gt; 10 months) expanded cloned CTL lines derived <jats:italic>in vitro<\/jats:italic> from an <jats:italic>in vivo<\/jats:italic> allosensitized individual exhibiting various specificities. Unexpectedly, only 10% of these CTL clones were inhibited. Flow cytofluorimetric analysis further revealed that noninhibited and inhibited CTL clones expressed similar amounts of the 30\u2010kDa structure. Consequently, the inhibition of CTL was heterogeneous when analyzed at the clonal level and not simply related to the presence or absence of this structure. Furthermore, the ability of CTL clones to be inhibited appeared to be unrelated to their HLA\u2010A, B or C specificity or to their lytic activity. The connections between this mAb\u2010defined structure and CTL function are discussed.<\/jats:p>","DOI":"10.1002\/eji.1830120908","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T19:00:53Z","timestamp":1172689253000},"page":"739-747","source":"Crossref","is-referenced-by-count":152,"title":["Human cytotoxic T cell structures associated with expression of cytolysis. I. Analysis at the clonal cell level of the cytolysis\u2010inhibiting effect of 7 monoclonal antibodies"],"prefix":"10.1002","volume":"12","author":[{"given":"Bernard","family":"Malissen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Najet","family":"Rebai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9","family":"Liabeuf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Mawas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/281307a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01567821"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0198-8859(81)90002-1"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.3.1858"},{"key":"e_1_2_1_6_2","volume-title":"Isolation, characterization and utilization of T lymphocyte clones","author":"Malissen B.","year":"1982"},{"key":"e_1_2_1_7_2","first-page":"182s","volume":"152","author":"Goulmy E.","year":"1980","journal-title":"J. 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