{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T10:52:08Z","timestamp":1758279128236},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":8743,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1982,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Splenocytes of nu\/nu mice treated with serum thymic factor (FTS) for 3 or more days followed by stimulation with either phorbol myristate acetate (PMA) or concanavalin A (Con A) produced interleukin 2 (IL2) as determined in two indicator systems, namely, support of growth of IL 2\u2010dependent T cells and promotion of Con A\u2010initiated mitogenesis of thymocytes. However, neither mitogens nor FTS alone could induce nude mice cells to produce IL2.<\/jats:p><jats:p>Supernatants derived from the tumor cell line WEHI\u20103 (WEHI\u20103 conditioned media) induced and supported continuous growth in culture of Thy\u20101.2<jats:sup>+<\/jats:sup>, Lyt\u20101<jats:sup>+<\/jats:sup>2<jats:sup>\u2212<\/jats:sup> lymphocytes from athymic nude mice capable of producing IL2 after their stimulation by either, PMA or Con A. The growth of these IL 2\u2010producer cell lines strickly depends on the presence of WEHI\u20103 conditioned media, as in the absence of it they die 24\u201048 h later. In addition, WEHI\u20103 conditioned media have been supporting the growth of IL 2\u2010producer cell lines derived from nude mice for 3 1\/2 months.<\/jats:p><jats:p>The helper factor contained in WEHI\u20103 conditioned media responsible for the above biological activity has an apparent mol. wt. of \u223c 40 000 as determined by Sephadex G\u2010100 chromatography and lacks IL1 and IL2 activities, but efficiently supports the growth of IL 2\u2010producer cells derived from nude mice and the peripheral blood of normal human volunteers. These results indicate that the helper factor in WEHI\u20103 conditioned media which enables the generation and continuous proliferation in culture of IL 2\u2010producer cells in nude mice is distinct from interleukin 1, IL2 and FTS (mol. wt. 864). Finally, the possible functional relationship of FTS and the helper factor produced by WEHI\u20103 cells is discussed.<\/jats:p>","DOI":"10.1002\/eji.1830120914","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T19:00:53Z","timestamp":1172689253000},"page":"777-782","source":"Crossref","is-referenced-by-count":14,"title":["Development and continuous growth in culture of interleukin 2\u2010producer lymphocytes from athymic nu\/nu mice"],"prefix":"10.1002","volume":"12","author":[{"given":"Ronald","family":"Palacios","sequence":"first","affiliation":[]},{"given":"Carmen","family":"Fernandez","sequence":"additional","affiliation":[]},{"given":"Paschalis","family":"Sideras","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.148.6.1523"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00342200"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.181845"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1980.tb00324.x"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1980.tb00327.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1982.tb00412.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/283664a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1980.tb00320.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.134.3.681"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/217370a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-0325-2_10"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.149.6.1460"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/284278a0"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.152.3.688"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830100912"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.151.6.1551"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.120.5.1504"},{"key":"e_1_2_1_19_2","first-page":"127","volume":"42","author":"Palacios R.","year":"1981","journal-title":"Immunology"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"8040","DOI":"10.1016\/S0021-9258(17)40932-X","volume":"252","author":"Dardenne M.","year":"1977","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_21_2","first-page":"208","volume-title":"Autoimmunity","author":"Bach J.\u2010F.","year":"1977"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/269597a0"},{"key":"e_1_2_1_23_2","first-page":"470","volume":"42","author":"Monier J. C.","year":"1981","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1982.tb00409.x"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90242-7"},{"key":"e_1_2_1_26_2","doi-asserted-by":"crossref","first-page":"852","DOI":"10.4049\/jimmunol.128.2.852","volume":"128","author":"Walker E. B.","year":"1982","journal-title":"J. Immunol."}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830120914","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830120914","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T07:15:23Z","timestamp":1698909323000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830120914"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,1]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1982,1]]}},"alternative-id":["10.1002\/eji.1830120914"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830120914","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,1]]}}}