{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T23:03:00Z","timestamp":1697842980413},"reference-count":16,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":8355,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1983,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antisera were raised in rabbits against L chains, isolated from mouse myeloma protein MOPC21 (\u03b3<jats:sub>1<\/jats:sub>,<jats:italic>x<\/jats:italic>, V<jats:sub><jats:italic>x<\/jats:italic>15<\/jats:sub> group). Specific antibodies for the V and C domain of MOPC 21 L chain were obtained by cross\u2010immunoadsorption of the antisera. The pure anti\u2010V and anti\u2010C antibodies were fixed on diazocellulose and used as immunosorbents. The inhibitory capacity of L chain\u2010monomers and dimers isolated from the L chain preparation was compared to that of intact IgG<jats:sub>1<\/jats:sub> using binding inhibition of <jats:sup>125<\/jats:sup>I\u2010labeled IgG<jats:sub>1<\/jats:sub> on the antibody\u2010containing immunosorbents. It was established that changes of IgG<jats:sub>1<\/jats:sub> quaternary structure influences the conformational state of the L chain V domain only. The inhibitory capacity of the V domain is 1000\u2010fold lower in L monomers, if compared with native IgG<jats:sub>1<\/jats:sub>, and only 10\u2010fold lower than in L dimers. The inhibiting capacity of the C domain, however, does not differ in L monomers and intact IgG<jats:sub>1<\/jats:sub>. Thus the conformational rigidity of the C domain co\u2010exists with conformational flexibility of the V domain on the same polypeptide chain. We tried to estimate the content of MOPC 21 V<jats:sub>L<\/jats:sub>\u2010like normal IgG in mouse serum of 6 inbred strains using antibodies against the V<jats:sub>L<\/jats:sub> domain. Data obtained by inhibition of radioimmunoadsorption, indicate that in C57BL\/6 mice 0.08% of normal serum Ig carries a V<jats:sub>L<\/jats:sub> region which is idiotypically related to the V<jats:sub>L<\/jats:sub> of MOPC 21. In serum Ig of BALB\/c mice the percentage is 0.16.<\/jats:p>","DOI":"10.1002\/eji.1830130509","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T20:23:09Z","timestamp":1172694189000},"page":"397-403","source":"Crossref","is-referenced-by-count":4,"title":["Antigenic markers of V domain of mouse MOPC 21 IgG<sub>1<\/sub> L chain. The conformational basis of V<sub>L<\/sub> domain markers and content of MOPC 21 V<sub>L<\/sub>\u2010like immunoglobulins in sera of inbred mouse strains"],"prefix":"10.1002","volume":"13","author":[{"given":"Oscar V.","family":"Rokhlin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margarita N.","family":"Petrosyan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander R.","family":"Ibraghimov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara I.","family":"Vengerova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Galina T.","family":"Bogachova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00594a022"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1093\/oxfordjournals.jbchem.a132058","volume":"83","author":"Azuma T.","year":"1978","journal-title":"J. Biochem."},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"1905","DOI":"10.4049\/jimmunol.122.5.1905","volume":"122","author":"Vrana M.","year":"1979","journal-title":"J. Immunol."},{"key":"e_1_2_1_5_2","first-page":"1303","volume":"13","author":"Vengerova T. I.","year":"1980","journal-title":"Mol. Biol. (USSR)"},{"key":"e_1_2_1_6_2","first-page":"1197","volume":"14","author":"Petrosyan M. N.","year":"1980","journal-title":"Mol. Biol. (USSR)"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1260837"},{"key":"e_1_2_1_8_2","first-page":"8.1","volume-title":"Handbook of Experimental Immunology","author":"Williamson A.","year":"1973"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)94333-4"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.63.1.71"},{"key":"e_1_2_1_11_2","first-page":"934","volume":"31","author":"Gurvich A. E.","year":"1961","journal-title":"Biokhimia (USSR)"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(71)90404-6"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-122-31148"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-635502-4.50008-4"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(81)90042-0"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830080109"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(81)90043-2"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830130509","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830130509","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T21:46:40Z","timestamp":1695764800000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830130509"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,1]]},"references-count":16,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1983,1]]}},"alternative-id":["10.1002\/eji.1830130509"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830130509","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,1]]}}}