{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:45:11Z","timestamp":1762271111073},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":8355,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1983,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mouse monoclonal antibodies W3\/25 and MRC OX\u20108 have been used to distinguish rat T lymphocyte subpopulations. In the peripheral T lymphocyte population, W3\/25 antibody recognizes an antigen on the T<jats:sub>helper<\/jats:sub> (T<jats:sub>h<\/jats:sub>) subset, while MRC OX\u20108 antibody recognizes an antigen on the T<jats:sub>suppressor\/cytotoxic<\/jats:sub> (T<jats:sub>s\/c<\/jats:sub>) subset. To determine the nature of these antigens, rat thymocytes were either metabolically labeled with [<jats:sup>35<\/jats:sup>S]<jats:sc>L<\/jats:sc>\u2010methionine or surface\u2010labeled at sialic acid residues by periodate oxidation followed by [<jats:sup>3<\/jats:sup>H]NaBH<jats:sub>4<\/jats:sub> reduction. Thymocytes were solubilized with nonionic detergent, the antigens immunoprecipitated and the molecular weights determined by sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis analysis. Using metabolically labeled cells, W3\/25 antibody immunoprecipitated an antigen that electrophoresed under reducing conditions as a broad band between 48000\u201353000 M<jats:sub>r<\/jats:sub>. Unreduced samples migrated between 46000\u201350000 M<jats:sub>r<\/jats:sub>. Surface\u2010labeled W3\/25 antigen, electrophoresed under reducing conditions, separated into two bands of 44000 and 52000 M<jats:sub>r<\/jats:sub>. Metabolically labeled MRC OX\u20108 antigen was identified as a protein of at least two chains of 39000 and 34000 M<jats:sub>r<\/jats:sub>. There was also a fainter band at approximately 67000 M<jats:sub>r<\/jats:sub>. Unreduced samples indicated a more complex structure with bands at 70000, 110000 and 165000 M<jats:sub>r<\/jats:sub>. Surface\u2010labeled MRC OX\u20108 antigen was of similar nature. These data, when considered with functional and tissue distribution data, suggest that W3\/25 antigen is equivalent to T4 in man and MRC OX\u20108 antigen is equivalent to T8 in man, and Lyt\u20102 in mouse.<\/jats:p>","DOI":"10.1002\/eji.1830131014","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T19:27:06Z","timestamp":1172690826000},"page":"855-858","source":"Crossref","is-referenced-by-count":29,"title":["Molecular nature of the W3\/25 and MRC OX\u20108 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens"],"prefix":"10.1002","volume":"13","author":[{"given":"Matthew L.","family":"Thomas","sequence":"first","affiliation":[]},{"given":"Jon R.","family":"Green","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.148.3.664"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830100807"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830120612"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(77)90266-5"},{"key":"e_1_2_1_6_2","first-page":"593","volume":"42","author":"Barclay A. 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