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Panning experiments, in which cells which initiate colony formation are depleted on plates coated with monoclonal antibodies, suggest that by the 13<jats:sup>th<\/jats:sup> day of gestation they express the antigen recognized by the monoclonal antibody AA4.1 and by day 14 they also express the B220 form of Ly\u20105 recognized by the monoclonal antibody 14.8. By similar criteria the precursor cells do not express \u03bc, I\u2010A, I\u2010E or Lyb\u20102. Growth of cells in this assay is dependent upon soluble products provided by either fetal liver adherent cells, bone marrow adherent cells or colony\u2010stimulating factor\u2010containing conditioned media derived from placenta cells, L929 cells, WEHI\u20103 B(D<jats:sup>\u2212<\/jats:sup>) cells, T helper cells or mouse lung cells. These experiments define two sets of growth conditions. In the first, when support is provided by fetal liver adherent cells, the limiting component appears to be the B cell precursor, allowing us to estimate the frequency of these cells during ontogeny. We find approximately 1 clonable pre\u2010B cell in 300000 fetal liver cells on day 12 of gestation and 1 in 6000 by day 16. Under the second set of growth conditions, when support is provided by bone marrow adherent cells or colony\u2010stimulating factor\u2010containing conditioned media, more than one cell, colony or cell product is limiting. Highly purified samples of granulocyte\/macrophage colony\u2010stimulating factor, colony\u2010stimulating factor 1 and multilineage\u2010hematopoietic growth factor are effective in this assay suggesting that the colony\u2010stimulating factors are the active components under these conditions.<\/jats:p>","DOI":"10.1002\/eji.1830141104","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T20:56:10Z","timestamp":1172696170000},"page":"979-987","source":"Crossref","is-referenced-by-count":39,"title":["Differentiation of murine B cell precursors in agar culture. 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