{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:16:47Z","timestamp":1758280607687},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,11,16]],"date-time":"2005-11-16T00:00:00Z","timestamp":1132099200000},"content-version":"vor","delay-in-days":7624,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1985,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Large sialoglycoprotein of human lymphocytes (L\u2010LSGP) from thymocytes and from peripheral blood lymphocytes (PBL) of normal donor and of B chronic lymphocytic leukemia (CLL) patients was purified by affinity chromatography to <jats:italic>Mucluru pornifera<\/jats:italic> agglutinin (MPA)\u2010Sepharose followed by preparative sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis (SDS\u2010PAGE). L\u2010LSGP from the three different sources was very similar in amino acid composition. It contained a high proportion of acidic and hydroxy amino acids and also significant amounts of cystein. No reduction in mobility in SDS\u2010PAGE was noted for unreduced L\u2010LSGP. The molecule may already in its native form have an extended conformation containing either free sulfhydryl groups or small S\u2010S loops not affecting mobility in SDS\u2010PAGE. L\u2010LSGP was found to be highly glycosylated, the thymocyte glycoprotein containing somewhat less carbohydrate by weight (44%) than that of PBL (normal PBL 53% and B CLL 52%). This was due primarily to a lower content of sialic acid. The molecules contained mannose, galactose, N\u2010acetyl galactosamine, N\u2010acetylglucosamine and sialic acid in molar ratios 1.0:3.0:1.1:1.2:1.3 (thymocyte L\u2010LSGP), 1.0:3.8:1.2:1.0:1.7 (PBL L\u2010LSGP) and 1.0:3.5:2.2:1.3:2.8 (B CLL L\u2010LSGP). The weak interaction of L\u2010LSGP with lentil lectin, concanavalin A (Con A) and leucoagglutinin (La), its unchanged mobility in SDS\u2010PAGE after tunicamycin treatment and its high amount of hydroxy amino acids suggest that most carbohydrate chains are O\u2010glycosidically linked to the peptide chain. Native as well as Nase\u2010treated L\u2010LSGP show size microheterogeneity. This is probably due to small chemical differences in the L\u2010LSGP molecules from different lymphocyte subsets.<\/jats:p>","DOI":"10.1002\/eji.1830150503","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T21:52:46Z","timestamp":1172699566000},"page":"427-433","source":"Crossref","is-referenced-by-count":13,"title":["The large sialoglycoprotein of human lymphocytes. II. 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