{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:38:14Z","timestamp":1758397094554},"reference-count":21,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":7630,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1985,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ability of two antibodies, one specific for the a chain, p180, and the other for the \u03b2 chain, p95, of the human lymphocyte function\u2010associated (LFA\u20101) antigens, to inhibit T cell function was measured. Both antibodies inhibited T cell\u2010mediated lysis of virus\u2010infected target cells and of K562 cells. Only the anti\u2010\u03b2 chain antibody inhibited natural killer cell lysis of K562. The antibodies inhibited cytotoxic T lymphocyte cell (CTL) lysis of HLA\u2010mismatched target cells in the presence of concanavalin A at 6.25\u201312.5 \u03bcg\/ml, but at higher doses of Con A no inhibition was seen. When the lytic process was divided into calcium\u2010independent (adherence) and \u2010dependent (lysis) steps the antibodies were found to block at the initial step of conjugate formation. The effects of these antibodies on T cell proliferative responses showed that responses to antigens, alloantigens, mitogens and anti\u2010CD3 (UCHT1) antibody were greatly inhibited. All of these responses are adherent cell dependent and proliferation of adherent cell\u2010depleted mononuclear cells to Sepharose\u2010coupled UCHT1 was not inhibited by anti\u2010LFA\u20101 antibodies. Proliferation to paired anti\u2010CD2 (T11) antibodies was also only weakly inhibited. Release of interferon\u2010\u03b3 by CTL on contact with target cells was also inhibited by anti\u2010LFA antibody. These results are evidence that the LFA antigen is necessary for a nonspecific interaction with antigen \u2010presenting cells that is essential for activation of T cells through the CD3\u2010T cell receptor complex.<\/jats:p>","DOI":"10.1002\/eji.1830150905","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T21:58:43Z","timestamp":1172699923000},"page":"888-892","source":"Crossref","is-referenced-by-count":41,"title":["Effects of monoclonal antibodies to the a and \u03b2 chains of the human lymphocyte function\u2010associated (H\u2010LFA\u20101) antigen on T lymphocyte functions"],"prefix":"10.1002","volume":"15","author":[{"given":"David W.","family":"Dongworth","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frances M.","family":"Gotch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James E. 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