{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T15:55:25Z","timestamp":1697990125787},"reference-count":9,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,11,22]],"date-time":"2005-11-22T00:00:00Z","timestamp":1132617600000},"content-version":"vor","delay-in-days":7265,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cell lineage of the lymphokine\u2010activated killer (LAK) cells has been reinvestigated. Both T and non\u2010T cells, isolated on the basis of rosette formation with sheep erythrocytes (E), generated LAK activity after 3\u20134 days of culture in recombinant interleukin 2 (rIL 2) in 8 different individuals tested. By applying a microculture technique which allows clonal expansion of virtually all E resetting T cells, we further analyzed the frequency of clonogenic LAK precursors within T cell populations. Approximately 1 of 25 T cells was found to be a LAK precursor. Moreover, microcultures with LAK activity lysed both the natural killer\u2010sensitive K562 cell line and the P815 target cells in the presence of phytohemagglutinin (PHA). Since cytolytic T lymphocytes capable of lysing P815 cells in the PHA\u2010dependent assay were approximately 1\/3, it is evident that only a minor subset of cytolytic T lymphocyte precursors can acquire LAK activity even in the presence of large amounts of IL 2. Several LAK clones obtained by limiting dilution were further expanded and analyzed for their phenotypic and functional properties. Twelve out of 14 clones analyzed expressed the T3<jats:sup>+<\/jats:sup> T11<jats:sup>+<\/jats:sup> phenotype whereas 2 were T3<jats:sup>\u2212<\/jats:sup> T11<jats:sup>+<\/jats:sup>. All had maintained their original cytolytic pattern; moreover, the large majority of the T3<jats:sup>+<\/jats:sup> clones produced IL 2 and interferon\u2010\u03b3 following PHA stimulation.<\/jats:p>","DOI":"10.1002\/eji.1830161224","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T22:41:48Z","timestamp":1172702508000},"page":"1623-1625","source":"Crossref","is-referenced-by-count":19,"title":["T cell nature of some lymphokine\u2010activated killer (LAK) cells. Frequency analysis of LAK precursors within human T cell populations and clonal analysis of LAK effector cells"],"prefix":"10.1002","volume":"16","author":[{"given":"Lorenzo","family":"Moretta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Pende","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raffaella","family":"Cozzani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalisa","family":"Merli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcello","family":"Bagnasco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Cristina","family":"Mingari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,11,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198512053132327"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.3.884"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.2.743"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.146.1.184"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.1985.36.3.337"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.158.2.571"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.4049\/jimmunol.120.6.2027","volume":"120","author":"Gillis S.","year":"1978","journal-title":"J. Immunol."},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/BF03029161","volume":"15","author":"Melioli S.","year":"1985","journal-title":"La Ricerca Clin. Lab."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.5.1284"}],"container-title":["European Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Feji.1830161224","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/eji.1830161224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T19:12:16Z","timestamp":1695755536000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/eji.1830161224"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":9,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/eji.1830161224"],"URL":"https:\/\/doi.org\/10.1002\/eji.1830161224","archive":["Portico"],"relation":{},"ISSN":["0014-2980","1521-4141"],"issn-type":[{"value":"0014-2980","type":"print"},{"value":"1521-4141","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}