{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,7]],"date-time":"2025-12-07T03:45:14Z","timestamp":1765079114498},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":6895,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Considerable numbers of large granular lymphocytes (LGL) were isolated from rat liver by a simple method consisting of sinusoidal lavage at elevated (50 cm water column) perfusion pressure. This method gave a yield comparable with the enzymatic dissociation method commonly used for the isolation of nonparenchymal liver cells, but was shorter in time and had the advantage of avoiding the potentially harmful effects of the dissociating enzymes. The isolated LGL were highly cytotoxic against YAC\u20101 lymphoma cells and this cytolytic activity was blocked by treatment of the effector cells with an antibody against natural killer cells (anti\u2010asialo GM<jats:sub>1<\/jats:sub>). We characterized the hepatic LGL as nonphagocytic, nonadherent, peroxidase\u2010negative and acid phosphatase\u2010positive cells which could be enriched in the low\u2010density fraction of a Percoll gradient. At the light microscopic level, they showed characteristic azurophilic granules which corresponded to strongly osmiophilic granules with a specific morphology in electron microscopy. It is concluded that these LGL are identical to the \u201cpit cells\u201d which were formerly described by electron microscopy <jats:italic>in situ<\/jats:italic> as normal components of the liver sinusoids and which are easily recognized by their fine structure. It is also proposed that the liver may represent one of the major natural killer organs.<\/jats:p>","DOI":"10.1002\/eji.1830170107","type":"journal-article","created":{"date-parts":[[2007,2,28]],"date-time":"2007-02-28T23:34:41Z","timestamp":1172705681000},"page":"37-42","source":"Crossref","is-referenced-by-count":114,"title":["Large granular lymphocytes or \u201cPit cells\u201d from rat liver: isolation, ultrastructural characterization and natural killer activity"],"prefix":"10.1002","volume":"17","author":[{"given":"Luc","family":"Bouwens","sequence":"first","affiliation":[]},{"given":"Linda","family":"Remels","sequence":"additional","affiliation":[]},{"given":"Marijke","family":"Baekeland","sequence":"additional","affiliation":[]},{"given":"Hans","family":"Van Bossuyt","sequence":"additional","affiliation":[]},{"given":"E.","family":"Wisse","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00224305"},{"key":"e_1_2_1_3_2","first-page":"77","volume-title":"Sinusoidal Liver Cells","author":"Kaneda K.","year":"1982"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00212219"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"567","DOI":"10.2220\/biomedres.4.567","volume":"4","author":"Kaneda K.","year":"1983","journal-title":"Biomed. 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