{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T18:44:38Z","timestamp":1698518678317},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,11,17]],"date-time":"2005-11-17T00:00:00Z","timestamp":1132185600000},"content-version":"vor","delay-in-days":6895,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Eur J Immunol"],"published-print":{"date-parts":[[1987,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The functional activity of Moloney murine sarcoma virus (M\u2010MSV)\u2010specific T lymphocytes <jats:italic>in vivo<\/jats:italic> was assayed by the i.v. injection of virus\u2010specific T lymphocytes into T cell\u2010deficient \u201cB mice\u201d. Virus\u2010specific T lymphocytes generated in mixed lymphocyte tumor cell cultures were transferred i.v. into syngeneic \u201cB mice\u201d injected simultaneously at a distant site with the virus. These experiments indicated that a low dose (1 \u00d7 10<jats:sup>6<\/jats:sup> cultured cells) of infused lymphocytes can afford protection. To define the T lymphocyte subpopulation which was active, Lyt\u20102<jats:sup>+<\/jats:sup> lymphocytes were selected by \u201cpanning\u201d on plastic petri dishes coated with anti\u2010Lyt\u20102 monoclonal antibody, and Lyt\u20102<jats:sup>\u2212<\/jats:sup> lymphocytes selected by treatment with anti\u2010Lyt\u20102 monoclonal antibody and complement. The results indicated that a Lyt\u20102<jats:sup>+<\/jats:sup> lymphocyte\u2010enriched population was more efficient in conferring protection against M\u2010MSV\u2010induced tumors.<\/jats:p><jats:p>To investigate if cytolytic T lymphocytes (CTL) alone had a protective effect, a M\u2010MSV\u2010specific CTL clone was transferred in the same model system. The results demonstrated that a M\u2010MSV\u2010specific CTL clone prevented M\u2010MSV\u2010induced tumor growth and also induced the destruction of syngeneic Moloney murine leukemia virus (M\u2010MuLV)\u2010induced MBL\u20102 leukemic cells in the peritoneal cavity. However, the cell dose required to obtain protection using a CTL clone was higher than that which was effective when mixed lymphocyte tumor cell culture cells were used.<\/jats:p><jats:p>To assess the ability of the transferred cells to home and to repopulate the lymphoid organs of the \u201cB mice\u201d, the frequency of virus\u2010specific CTL precursors in the spleen was evaluated by limiting dilution analysis. The results indicated that lymphocytes from mixed lymphocyte tumor cell cultures can be recovered from the spleens of \u201cB mice\u201d injected i.v. 25 days earlier. On the contrary, following the transfer of an active CTL clone, a very low frequency (&lt; 1\/200000 cells) of virus\u2010specific CTL precursors was present in the spleens of recipient animals. The same M\u2010MSV\u2010specific CTL clone did not yield protection against M\u2010MSV\u2010induced tumors or MBL\u20102 leukemic cells when injected i.v. into M\u2010MuLV tolerant mice.<\/jats:p>","DOI":"10.1002\/eji.1830170204","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:31:01Z","timestamp":1172709061000},"page":"173-178","source":"Crossref","is-referenced-by-count":11,"title":["Functional activity <i>in vivo<\/i> of effector T cell populations III. Protection against Moloney murine sarcoma virus (M\u2010MSV)\u2010induced tumors in T cell deficient mice by the adoptive transfer of a M\u2010MSV\u2010specific cytolytic T lymphocyte clone"],"prefix":"10.1002","volume":"17","author":[{"given":"Vincenzo","family":"Cerundolo","sequence":"first","affiliation":[]},{"given":"Thierry","family":"Lahaye","sequence":"additional","affiliation":[]},{"given":"Clotilde","family":"Horvath","sequence":"additional","affiliation":[]},{"given":"Paola","family":"Zanovello","sequence":"additional","affiliation":[]},{"given":"Dino","family":"Collavo","sequence":"additional","affiliation":[]},{"given":"Howard D.","family":"Engers","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.152.4.823"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198202000-00029"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.1984.tb00717.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.4049\/jimmunol.133.3.1664","volume":"133","author":"Engers H. 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