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IgA, isolated from normal human serum, was aggregated by either N\u2010succinimidyl 3\u2010(2\u2010pyridyldithio)propionate (SPDP), glutaraldehyde, carbodiimide or heating. Depending on the size of the aggregates, and on the method of aggregation, E coated with aggregated IgA (E\u03b3<jats:sub>1<\/jats:sub>.AIgA) could be lysed. The alternative pathway of complement appeared to mediate the lysis because the latter was observed in the presence of EGTA containing 5 mM Mg<jats:sup>2+<\/jats:sup> (MgEGTA) and properdin (P) was deposited on the cells. Furthermore, no lysis was observed in C3\u2010deficient serum. In the absence of AIgA the cells were not lysed, and no P deposition was observed. In another set of experiments E\u03b3<jats:sub>1<\/jats:sub>.AIgA were first reacted with purified C3, B, D and P for 30 min at 30\u00b0C, and subsequently in rat serum EDTA at 37\u00b0C. Lysis occurred when E\u03b3<jats:sub>1<\/jats:sub>.AIgA were prepared using SPDP\u2010, glutaraldehyde\u2010 or carbodiimide\u2010AIgA. Incubation of 100 \u03bcg\/ml SPDP\u2010AIgA with normal human serum for 30 min at 37\u00b0C in the presence or absence of MgEGTA also induced consumption of total complement. The other soluble AIgA preparations were less effective in activating complement. These results suggest that polymeric serum IgA is capable of activating the alternative pathway of complement.<\/jats:p>","DOI":"10.1002\/eji.1830170304","type":"journal-article","created":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:30:33Z","timestamp":1172709033000},"page":"321-326","source":"Crossref","is-referenced-by-count":145,"title":["Activation of the alternative pathway of complement by human serum IgA"],"prefix":"10.1002","volume":"17","author":[{"given":"Pieter S.","family":"Hiemstra","sequence":"first","affiliation":[]},{"given":"Arko","family":"Gorter","sequence":"additional","affiliation":[]},{"given":"Marly E.","family":"Stuurman","sequence":"additional","affiliation":[]},{"given":"Leendert A.","family":"Van Es","sequence":"additional","affiliation":[]},{"given":"Mohamed R.","family":"Daha","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2005,11,17]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"939","volume":"1","author":"Berger J.","year":"1969","journal-title":"Transplant. 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